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Type 1 Diabetes Mellitus and the First Trimester Placenta: Hyperglycemia-Induced Effects on Trophoblast Proliferation, Cell Cycle Regulators, and Invasion
International Journal of Molecular Sciences, 22 (2021), 20; 10989. https://doi.org/10.3390/ijms222010989
Majali-Martinez, Alejandro; Weiss-Fuchs, Ursula; Miedl, Heidi; Forstner, Desiree; Bandres-Meriz, Julia; Hoch, Denise; Djelmis, Josip; Ivanišević, Marina; Hiden, Ursula; Gauster, Martin; Desoye, Gernot

Institucijski repozitorij: Dr Med - digitalni repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

Citirajte ovaj rad

Majali Martinez, A., Weiss Fuchs, U., Miedl, H., Forstner, D., Bandres Meriz, J., Hoch, D. ... Desoye, G. (2021). Type 1 Diabetes Mellitus and the First Trimester Placenta: Hyperglycemia-Induced Effects on Trophoblast Proliferation, Cell Cycle Regulators, and Invasion. International Journal of Molecular Sciences, 22. (20). doi: 10.3390/ijms222010989

Majali Martinez, Alejandro, et al. "Type 1 Diabetes Mellitus and the First Trimester Placenta: Hyperglycemia-Induced Effects on Trophoblast Proliferation, Cell Cycle Regulators, and Invasion." International Journal of Molecular Sciences, vol. 22, br. 20, 2021. https://doi.org/10.3390/ijms222010989

Majali Martinez, Alejandro, Ursula Weiss Fuchs, Heidi Miedl, Desiree Forstner, Julia Bandres Meriz, Denise Hoch, Josip Djelmis, et al. "Type 1 Diabetes Mellitus and the First Trimester Placenta: Hyperglycemia-Induced Effects on Trophoblast Proliferation, Cell Cycle Regulators, and Invasion." International Journal of Molecular Sciences 22, br. 20 (2021). https://doi.org/10.3390/ijms222010989

Majali Martinez, A., et al. (2021) 'Type 1 Diabetes Mellitus and the First Trimester Placenta: Hyperglycemia-Induced Effects on Trophoblast Proliferation, Cell Cycle Regulators, and Invasion', International Journal of Molecular Sciences, 22(20). doi: 10.3390/ijms222010989

Majali Martinez A, Weiss Fuchs U, Miedl H, Forstner D, Bandres Meriz J, Hoch D, i sur.. Type 1 Diabetes Mellitus and the First Trimester Placenta: Hyperglycemia-Induced Effects on Trophoblast Proliferation, Cell Cycle Regulators, and Invasion. International Journal of Molecular Sciences [Internet]. 12.10.2021. [pristupljeno 01.12.2024.];22(20). doi: 10.3390/ijms222010989

A. Majali Martinez, et al., "Type 1 Diabetes Mellitus and the First Trimester Placenta: Hyperglycemia-Induced Effects on Trophoblast Proliferation, Cell Cycle Regulators, and Invasion", International Journal of Molecular Sciences, vol. 22, br. 20, Listopad 2021. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:979714. [Citirano: 01.12.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:105:979714
Podaci o radu
Naslov (engleski)Type 1 Diabetes Mellitus and the First Trimester Placenta: Hyperglycemia-Induced Effects on Trophoblast Proliferation, Cell Cycle Regulators, and Invasion
AutorAlejandro Majali-Martinez
AutorUrsula Weiss-Fuchs
AutorHeidi Miedl
AutorDesiree Forstner
AutorJulia Bandres-Meriz
AutorDenise Hoch
AutorJosip Djelmis
AutorMarina Ivanišević
AutorUrsula Hiden
AutorMartin Gauster
AutorGernot Desoye
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA GINEKOLOGIJU I OPSTETRICIJU)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Ginekologija i opstetricija
Sažetak (engleski)
Type 1 diabetes mellitus (T1DM) is associated with reduced fetal growth in early pregnancy, but a contributing role of the placenta has remained elusive. Thus, we investigated whether T1DM alters placental development in the first trimester. Using a protein array, the level of 60 cell-cycle-related proteins was determined in human first trimester placental tissue (gestational week 5–11) from control (n = 11) and T1DM pregnancies (n = 12). Primary trophoblasts (gestational week 7–12, n = 32) were incubated in the absence (control) or presence of hyperglycemia (25 mM D-glucose) and hyperosmolarity (5.5 mM D-glucose + 19.5 mM D-mannitol). We quantified the number of viable and dead trophoblasts (CASY Counter) and assessed cell cycle distribution (FACS) and trophoblast invasion using a transwell assay. T1DM was associated with a significant (p < 0.05) downregulation of Ki67 (−26%), chk1 (−25%), and p73 (−26%). The number of viable trophoblasts was reduced under hyperglycemia (−23%) and hyperosmolarity (−18%), whereas trophoblast invasion was increased only under hyperglycemia (+6%). Trophoblast cell death and cell cycle distribution remained unaffected. Collectively, our data demonstrate that hyperglycemia decreases trophoblast proliferation as a potential contributing factor to the reduced placental growth in T1DM in vivo.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaInternational Journal of Molecular Sciences
Brojčani podacivol. 22, br. 20, 10989
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms222010989
URN:NBNurn:nbn:hr:105:979714
Datum objave publikacije2021-10-12
URL dokumentahttps://www.mdpi.com/1422-0067/22/20/10989
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2022-04-01 10:03:43