In order to develop a better therapeutic solutions for bone regeneration, a new osteoinductive device has been developed consisting of rhBMP6 and autologous blood coagulum as a carrier, which is currently being tested in clinical trials as a new therapeutic solution for bone repair and regeneration. Part of this research was focused on the investigation of the biology and time course of ectopic bone formation induced by rhBMP6 in autologous blood coagulum (ABC) and compared with the gold standard for bone healing, rhBMP2 on absorbable collagen sponge (ACS), where both osteoinductive devices were evaluated in the rat subcutaneous assay. Also, in a series of in vitro experiments release dynamics of both osteoinductive devices was tested showing that absorbable collagen sponge does not have a satisfactory release profile. In order to examine the cell populations involved in early osteogenesis (24h and 72h after subcutaneous implantation) induced by the aforementioned osteoinductive devices, the number of lymphoid, myeloid and mesenchymal cell populations were analyzed. Adipoprogenitors were most abundant in rhBMP2/ACS implants, while mesenchimal stem cells were predominately present in rhBMP6/ABC implants. Furthermore, the superiority of rhBMP6/ABC over rhBMP2/ACS was demonstrated in a rat subcutaneous assay, where at all observed time points, the largest amount of newly formed bone was induced by rhBMP6 / ABC at a dose of 20 μg. It has been observed that the absorbable collagen sponge as a carrier at the ectopic site degrades more slowly compared to the autologous blood coagulum. Consequently, the ossification process is delayed when rhBMP2/ACS is used due to the dense collagen meshwork that slows down the entry of progenitor cells, and thus the ossification process is delayed in comparison to the blood coagulum. In order to test the efficacy of the recombinant proteins used, rhBMP2 and rhBMP6, their osteoinductive potential was tested on an equal scaffold consisting of autologous blood coagulum and biphasic ceramics in the particle size range of 500 to 1700 μm in three different doses (5, 20, 50 μg), where rhBMP6 was superior to rhBMP2 at a dose of 50 μg. A potential reason for this is already proven resistance of rhBMP6 to noggin inhibition. Also, the addition of biocompatible ceramics as a compression-resistant matrix improves the biomechanical properties of implants, and the process of ossification of osteoinductive implants with the addition of ceramic particles is different compared to the blood coagulum itself.