prikaz prve stranice dokumenta Valproate Targets Mammalian Gastrulation Impairing Neural Tissue Differentiation and Development of the Placental Source In Vitro
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Valproate Targets Mammalian Gastrulation Impairing Neural Tissue Differentiation and Development of the Placental Source In Vitro
International Journal of Molecular Sciences, 23 (2022), 16; 8861. https://doi.org/10.3390/ijms23168861
Katušić-Bojanac, Ana; Plazibat, Milvija; Himelreich-Perić, Marta; Eck-Raković, Katarina; Krasić, Jure; Sinčić, Nino; Jurić-Lekić, Gordana; Ježek, Davor; Bulić-Jakuš, Floriana

Institucijski repozitorij: Dr Med - digitalni repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

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Katušić Bojanac, A., Plazibat, M., Himelreich Perić, M., Eck Raković, K., Krasić, J., Sinčić, N. ... Bulić Jakuš, F. (2022). Valproate Targets Mammalian Gastrulation Impairing Neural Tissue Differentiation and Development of the Placental Source In Vitro. International Journal of Molecular Sciences, 23. (16). doi: 10.3390/ijms23168861

Katušić Bojanac, Ana, et al. "Valproate Targets Mammalian Gastrulation Impairing Neural Tissue Differentiation and Development of the Placental Source In Vitro." International Journal of Molecular Sciences, vol. 23, br. 16, 2022. https://doi.org/10.3390/ijms23168861

Katušić Bojanac, Ana, Milvija Plazibat, Marta Himelreich Perić, Katarina Eck Raković, Jure Krasić, Nino Sinčić, Gordana Jurić Lekić, Davor Ježek i Floriana Bulić Jakuš. "Valproate Targets Mammalian Gastrulation Impairing Neural Tissue Differentiation and Development of the Placental Source In Vitro." International Journal of Molecular Sciences 23, br. 16 (2022). https://doi.org/10.3390/ijms23168861

Katušić Bojanac, A., et al. (2022) 'Valproate Targets Mammalian Gastrulation Impairing Neural Tissue Differentiation and Development of the Placental Source In Vitro', International Journal of Molecular Sciences, 23(16). doi: 10.3390/ijms23168861

Katušić Bojanac A, Plazibat M, Himelreich Perić M, Eck Raković K, Krasić J, Sinčić N, i sur.. Valproate Targets Mammalian Gastrulation Impairing Neural Tissue Differentiation and Development of the Placental Source In Vitro. International Journal of Molecular Sciences [Internet]. 09.08.2022. [pristupljeno 03.01.2025.];23(16). doi: 10.3390/ijms23168861

A. Katušić Bojanac, et al., "Valproate Targets Mammalian Gastrulation Impairing Neural Tissue Differentiation and Development of the Placental Source In Vitro", International Journal of Molecular Sciences, vol. 23, br. 16, Kolovoz 2022. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:001479. [Citirano: 03.01.2025.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:105:001479
Podaci o radu
Naslov (engleski)Valproate Targets Mammalian Gastrulation Impairing Neural Tissue Differentiation and Development of the Placental Source In Vitro
AutorAna Katušić-Bojanac
AutorMilvija Plazibat
AutorMarta Himelreich-Perić
AutorKatarina Eck-Raković
AutorJure Krasić
AutorNino Sinčić
AutorGordana Jurić-Lekić
AutorDavor Ježek
AutorFloriana Bulić-Jakuš
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA HISTOLOGIJU I EMBRIOLOGIJU)
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA MEDICINSKU BIOLOGIJU)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Citologija, histologija i embriologija
Sažetak (engleski)
The teratogenic activity of valproate (VPA), an antiepileptic and an inhibitor of histone deacetylase (HDACi), is dose-dependent in humans. Previous results showed that VPA impairs in vitro development and neural differentiation of the gastrulating embryo proper. We aimed to investigate the impact of a lower VPA dose in vitro and whether this effect is retained in transplants in vivo. Rat embryos proper (E9.5) and ectoplacental cones were separately cultivated at the air-liquid interface with or without 1 mM VPA. Embryos were additionally cultivated with HDACi Trichostatin A (TSA), while some cultures were syngeneically transplanted under the kidney capsule for 14 days. Embryos were subjected to routine histology, immunohistochemistry, Western blotting and pyrosequencing. The overall growth of VPA-treated embryos in vitro was significantly impaired. However, no differences in the apoptosis or proliferation index were found. Incidence of the neural tissue was lower in VPA-treated embryos than in controls. TSA also impaired growth and neural differentiation in vitro. VPA-treated embryos and their subsequent transplants expressed a marker of undifferentiated neural cells compared to controls where neural differentiation markers were expressed. VPA increased the acetylation of histones. Our results point to gastrulation as a sensitive period for neurodevelopmental impairment caused by VPA.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaInternational Journal of Molecular Sciences
Brojčani podacivol. 23, br. 16, 8861
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms23168861
URN:NBNurn:nbn:hr:105:001479
Datum objave publikacije2022-08-09
URL dokumentahttps://www.mdpi.com/1422-0067/23/16/8861
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2023-03-23 12:38:54