prikaz prve stranice dokumenta Splice variant of mouse Stam2 mRNA in nervous and muscle tissue contains additional exon with stop codon within region coding for VHS domain
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Splice variant of mouse Stam2 mRNA in nervous and muscle tissue contains additional exon with stop codon within region coding for VHS domain
Croatian Medical Journal, 47 (2006), 1; 16-24. urn:nbn:hr:105:295140
Ćurlin, Marija; Lucić, Vedran; Gajović, Vedran

Institucijski repozitorij: Dr Med - digitalni repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

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Ćurlin, M., Lucić, V. i Gajović, V. (2006). Splice variant of mouse Stam2 mRNA in nervous and muscle tissue contains additional exon with stop codon within region coding for VHS domain. Croatian Medical Journal, 47. (1), 16-24. Preuzeto s https://urn.nsk.hr/urn:nbn:hr:105:295140

Ćurlin, Marija, et al. "Splice variant of mouse Stam2 mRNA in nervous and muscle tissue contains additional exon with stop codon within region coding for VHS domain." Croatian Medical Journal, vol. 47, br. 1, 2006, str. 16-24. https://urn.nsk.hr/urn:nbn:hr:105:295140

Ćurlin, Marija, Vedran Lucić i Vedran Gajović. "Splice variant of mouse Stam2 mRNA in nervous and muscle tissue contains additional exon with stop codon within region coding for VHS domain." Croatian Medical Journal 47, br. 1 (2006): 16-24. https://urn.nsk.hr/urn:nbn:hr:105:295140

Ćurlin, M., Lucić, V. i Gajović, V. (2006) 'Splice variant of mouse Stam2 mRNA in nervous and muscle tissue contains additional exon with stop codon within region coding for VHS domain', Croatian Medical Journal, 47(1), str. 16-24. Preuzeto s: https://urn.nsk.hr/urn:nbn:hr:105:295140 (Datum pristupa: 04.01.2025.)

Ćurlin M, Lucić V, Gajović V. Splice variant of mouse Stam2 mRNA in nervous and muscle tissue contains additional exon with stop codon within region coding for VHS domain. Croatian Medical Journal [Internet]. 02.2006. [pristupljeno 04.01.2025.];47(1):16-24. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:295140

M. Ćurlin, V. Lucić i V. Gajović, "Splice variant of mouse Stam2 mRNA in nervous and muscle tissue contains additional exon with stop codon within region coding for VHS domain", Croatian Medical Journal, vol. 47, br. 1, str. 16-24, Veljača 2006. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:295140. [Citirano: 04.01.2025.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:105:295140
Podaci o radu
Naslov (engleski)Splice variant of mouse Stam2 mRNA in nervous and muscle tissue contains additional exon with stop codon within region coding for VHS domain
AutorMarija Ćurlin
AutorVedran Lucić
AutorVedran Gajović
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
AIM: To analyze the alternatively spliced variant of Stam2 mRNA and determine its distribution in mouse tissues. - - - - - METHODS: We identified a novel alternatively spliced exon by cloning and sequencing of Stam2 cDNA obtained from tissue samples of 3-5 months old male C57Bl/6NCrl mice. The sequence of the alternatively spliced exon was analyzed by bioinformatic tools. The tissue distribution of different Stam2 mRNA variants was determined by reverse transcription-polymerase chain reaction, and the consequences of the alternative splicing at the protein level were analyzed by western blot with the polyclonal anti-STAM2 antibody. - - - - - RESULTS: The novel alternatively spliced exon 1A of mouse Stam2 gene was inserted within Stam2 coding region and it contained a stop codon. The exon did not bear similarities to any other cDNA or protein sequence in the mouse, rat, or human databases. Both mRNA variants, with and without exon 1A, were present in the cortex, hippocampus, olfactory bulb, medulla oblongata, spinal cord, cerebellum, and the skeletal and heart muscle, while the other analyzed organs contained only the variant without the additional exon. The mRNA with the included exon did not give rise to a protein form detectable by western blotting with the polyclonal anti-STAM2 antibody. - - - - - CONCLUSION: The alternatively spliced exon 1A was included in mRNA splice variant present in the nervous and muscle tissues. The alternative splicing event did not have major impact on STAM2 production and functionality. It seems that exon 1A is an evolutionary new exon created by exonization of an intronic sequence.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaCroatian Medical Journal
Brojčani podacivol. 47, br. 1, str. 16-24
p-ISSN0353-9504
URN:NBNurn:nbn:hr:105:295140
Datum objave publikacije2006-02
URL dokumentahttp://www.cmj.hr/2006/47/1/16489693.htm
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2024-03-28 09:04:11