prikaz prve stranice dokumenta Impact of surface functionalization on the uptake mechanism and toxicity effects of silver nanoparticles in HepG2 cells
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Impact of surface functionalization on the uptake mechanism and toxicity effects of silver nanoparticles in HepG2 cells
Food and Chemical Toxicology, 107 (2017), Pt. A; 349-361. https://doi.org/10.1016/j.fct.2017.07.016
Brkić Ahmed, Lada; Milić, Mirta; Pongrac, Igor M.; Marjanović, Ana Marija; Mlinarić, Hrvoje; Pavičić, Ivan; Gajović, Srećko; Vinković Vrček, Ivana

Institucijski repozitorij: Dr Med - digitalni repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

Citirajte ovaj rad

Brkić Ahmed, L., Milić, M., Pongrac, I. M., Marjanović, A. M., Mlinarić, H., Pavičić, I. ... Vinković Vrček, I. (2017). Impact of surface functionalization on the uptake mechanism and toxicity effects of silver nanoparticles in HepG2 cells. Food and Chemical Toxicology, 107. (Pt. A), 349-361. doi: 10.1016/j.fct.2017.07.016

Brkić Ahmed, Lada, et al. "Impact of surface functionalization on the uptake mechanism and toxicity effects of silver nanoparticles in HepG2 cells." Food and Chemical Toxicology, vol. 107, br. Pt. A, 2017, str. 349-361. https://doi.org/10.1016/j.fct.2017.07.016

Brkić Ahmed, Lada, Mirta Milić, Igor M. Pongrac, Ana Marija Marjanović, Hrvoje Mlinarić, Ivan Pavičić, Srećko Gajović i Ivana Vinković Vrček. "Impact of surface functionalization on the uptake mechanism and toxicity effects of silver nanoparticles in HepG2 cells." Food and Chemical Toxicology 107, br. Pt. A (2017): 349-361. https://doi.org/10.1016/j.fct.2017.07.016

Brkić Ahmed, L., et al. (2017) 'Impact of surface functionalization on the uptake mechanism and toxicity effects of silver nanoparticles in HepG2 cells', Food and Chemical Toxicology, 107(Pt. A), str. 349-361. doi: 10.1016/j.fct.2017.07.016

Brkić Ahmed L, Milić M, Pongrac IM, Marjanović AM, Mlinarić H, Pavičić I, i sur.. Impact of surface functionalization on the uptake mechanism and toxicity effects of silver nanoparticles in HepG2 cells. Food and Chemical Toxicology [Internet]. 09.2017. [pristupljeno 29.11.2024.];107(Pt. A):349-361. doi: 10.1016/j.fct.2017.07.016

L. Brkić Ahmed, et al., "Impact of surface functionalization on the uptake mechanism and toxicity effects of silver nanoparticles in HepG2 cells", Food and Chemical Toxicology, vol. 107, br. Pt. A, str. 349-361, Rujan 2017. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:037776. [Citirano: 29.11.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:105:037776
Podaci o radu
Naslov (engleski)Impact of surface functionalization on the uptake mechanism and toxicity effects of silver nanoparticles in HepG2 cells
AutorLada Brkić Ahmed
AutorMirta Milić
AutorIgor M. Pongrac
AutorAna Marija Marjanović
AutorHrvoje Mlinarić
AutorIvan Pavičić
AutorSrećko Gajović
AutorIvana Vinković Vrček
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
Safe and successful bioapplications of metallic nanoparticles depend on their physicochemical characteristics, in particular their surface properties. This study aimed to investigate how different surface functionalization of silver nanoparticles (AgNP) affect their interaction with mammalian liver cells with regard to cytotoxicity, genotoxicity and mechanism of cellular uptake. Differentially coated AgNP were prepared by surface functionalization using sodium bis(2-ethylhexyl)-sulfosuccinate (AOTAgNP), cetyltrimethylammonium bromide (CTABAgNP), poly(vinylpyrrolidone) (PVPAgNP), poly-l-lysine (PLLAgNP), and bovine serum albumin (BSAAgNP). Data showed varying toxic potential of differentially coated AgNP. All AgNP types demonstrated concentration dependent effects on cytotoxicity and genotoxicity in HepG2 cells. Cytotoxic potential of differentially coated AgNP followed the order of BSAAgNP > PLLAgNP > CTABAgNP > AOTAgNP > PVPAgNP. Exposure of HepG2 cells to non-cytotoxic concentrations (up to 10 mg Ag/L) of AgNP for 24 h induced primary DNA damage as evaluated by alkaline comet assay. The highest increase in both comet tail length and tail intensity was produced by PLLAgNP followed by AOTAgNP, while CTABAgNP appeared to be least damaging. The main uptake mechanisms of AgNP were macropinocytosis and clathrin-mediated endocytosis. The study findings contribute to the criteria that should be considered in evaluating the biocompatibility and safety of novel nanomaterials.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeZavršna verzija rukopisa prihvaćena za objavljivanje (postprint)
Naslov časopisaFood and Chemical Toxicology
Brojčani podacivol. 107, br. Pt. A, str. 349-361
p-ISSN0278-6915
DOIhttps://doi.org/10.1016/j.fct.2017.07.016
URN:NBNurn:nbn:hr:105:037776
Datum objave publikacije2017-09
URL dokumentahttps://doi.org/10.1016/j.fct.2017.07.016
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2024-03-28 09:13:52