The aim of the study was to show whether there is a link between alcohol and drugs consumption, level of the education, night work and quality of life. For this study questionnaire of the Croatian Institute of health protection and safety at work was used with their permission. The participants answered questions about their workplace, alcohol and drug consumption, character of their work and perception of ther free time and quality of life. In the study there were 860 people, thereof 688 male and 168 female workers in different types of workplaces. The study was conducted in Republic of Croatia in period between March 2014 and July 2014. Out of 860 respondents 19 of them answered positively on the question whether they use marijuana or other psychoactive substances. There is no significant difference in drugs consumption among the work organizations. The results show that there is statistically significant difference in the frequency of alcohol usage among work organizations (p<0.001) and the difference in consumption between the sexes (p<0.001). There is also statistically significant difference between employees working in night shifts and their perception of the quality of life in relation to the number of night shifts per month (p<0.001). The largest number of workers who drink alcohol are workers with university degrees. Alcohol consumption is associated with their own perception of general health. There is statistically significant difference in alcohol consumption between workes who consider their health bad, compared to the health of workers who are considered good or excellent (p<0.001). Results of the study indicate the need for education of workers about the harmful effects of alcohol and drugs on their health and a multidisciplinary approach to this problem.