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BMP-6 exerts its osteoinductive effect through activation of IGF-I and EGF pathways
International Orthopaedics, 31 (2007), 6; 759-765. https://doi.org/10.1007/s00264-007-0407-9
Grasser, William A.; Orlić, Iva; Borovečki, Fran; Riccardi, K. A.; Šimić, Petra; Vukičević, Slobodan; Paralkar, Vishwas M.

Institucijski repozitorij: Dr Med - digitalni repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

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Grasser, W. A., Orlić, I., Borovečki, F., Riccardi, K. A., Šimić, P., Vukičević, S. i Paralkar, V. M. (2007). BMP-6 exerts its osteoinductive effect through activation of IGF-I and EGF pathways. International Orthopaedics, 31. (6), 759-765. doi: 10.1007/s00264-007-0407-9

Grasser, William A., et al. "BMP-6 exerts its osteoinductive effect through activation of IGF-I and EGF pathways." International Orthopaedics, vol. 31, br. 6, 2007, str. 759-765. https://doi.org/10.1007/s00264-007-0407-9

Grasser, William A., Iva Orlić, Fran Borovečki, K. A. Riccardi, Petra Šimić, Slobodan Vukičević i Vishwas M. Paralkar. "BMP-6 exerts its osteoinductive effect through activation of IGF-I and EGF pathways." International Orthopaedics 31, br. 6 (2007): 759-765. https://doi.org/10.1007/s00264-007-0407-9

Grasser, W. A., et al. (2007) 'BMP-6 exerts its osteoinductive effect through activation of IGF-I and EGF pathways', International Orthopaedics, 31(6), str. 759-765. doi: 10.1007/s00264-007-0407-9

Grasser WA, Orlić I, Borovečki F, Riccardi KA, Šimić P, Vukičević S, i sur.. BMP-6 exerts its osteoinductive effect through activation of IGF-I and EGF pathways. International Orthopaedics [Internet]. 12.2007. [pristupljeno 22.11.2024.];31(6):759-765. doi: 10.1007/s00264-007-0407-9

W. A. Grasser, et al., "BMP-6 exerts its osteoinductive effect through activation of IGF-I and EGF pathways", International Orthopaedics, vol. 31, br. 6, str. 759-765, Prosinac 2007. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:674422. [Citirano: 22.11.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:105:674422
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Naslov (engleski)BMP-6 exerts its osteoinductive effect through activation of IGF-I and EGF pathways
AutorWilliam A. Grasser
AutorIva Orlić
AutorFran Borovečki
AutorK. A. Riccardi
AutorPetra Šimić
AutorSlobodan Vukičević
AutorVishwas M. Paralkar
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
We have recently shown that human recombinant BMP-6 (rhBMP-6), given systematically, can restore bone in animal models of osteoporosis. To further elucidate the underlying mechanisms of new bone formation following systemic application of BMPs, we conducted gene expression profiling experiments using bone samples of oophrectomised mice treated with BMP-6. Gene set enrichment analysis revealed enrichment of insulin-like growth factor-I and epidermal growth factor related pathways in animals treated with BMP-6. Significant upregulation of IGF-I and EGF expression in bones of BMP-6 treated mice was confirmed by quantitative PCR. To develop an in vitro model for evaluation of the effects of BMP-6 on cells of human origin, we cultured primary human osteoblasts. Treatment with rhBMP-6 accelerated cell differentiation as indicated by the formation of mineralised nodules by day 18 of culture versus 28-30 days in vehicle treated cultures. In addition, alkaline phosphatase gene expression and activity were dramatically increased upon BMP-6 treatment. Expression of IGF-I and EGF was upregulated in human osteoblast cells treated with BMP-6. These results collectively indicate that BMP-6 exerts its osteoinductive effect, at least in part, through IGF-I and EGF pathways, which can be observed both in a murine model of osteopenia and in human osteoblasts.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeZavršna verzija rukopisa prihvaćena za objavljivanje (postprint)
Naslov časopisaInternational Orthopaedics
Brojčani podacivol. 31, br. 6, str. 759-765
p-ISSN0341-2695
DOIhttps://doi.org/10.1007/s00264-007-0407-9
URN:NBNurn:nbn:hr:105:674422
Datum objave publikacije2007-12
URL dokumentahttps://doi.org/10.1007/s00264-007-0407-9
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2024-03-28 09:27:16