prikaz prve stranice dokumenta Copresentation of BMP-6 and RGD ligands enhances cell adhesion and BMP-mediated signaling
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Copresentation of BMP-6 and RGD ligands enhances cell adhesion and BMP-mediated signaling
Cells, 8 (2019), 12; https://doi.org/10.3390/cells8121646
Posa, Francesca; Grab, Anna Luise; Martin, Volker; Hose, Dirk; Seckinger, Anja; Mori, Giorgio; Vukičević, Slobodan; Cavalcanti-Adam, Elisabetta Ada

Institucijski repozitorij: Dr Med - digitalni repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

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Posa, F., Grab, A. L., Martin, V., Hose, D., Seckinger, A., Mori, G. ... Cavalcanti Adam, E. A. (2019). Copresentation of BMP-6 and RGD ligands enhances cell adhesion and BMP-mediated signaling. Cells, 8. (12). doi: 10.3390/cells8121646

Posa, Francesca, et al. "Copresentation of BMP-6 and RGD ligands enhances cell adhesion and BMP-mediated signaling." Cells, vol. 8, br. 12, 2019. https://doi.org/10.3390/cells8121646

Posa, Francesca, Anna Luise Grab, Volker Martin, Dirk Hose, Anja Seckinger, Giorgio Mori, Slobodan Vukičević i Elisabetta Ada Cavalcanti Adam. "Copresentation of BMP-6 and RGD ligands enhances cell adhesion and BMP-mediated signaling." Cells 8, br. 12 (2019). https://doi.org/10.3390/cells8121646

Posa, F., et al. (2019) 'Copresentation of BMP-6 and RGD ligands enhances cell adhesion and BMP-mediated signaling', Cells, 8(12). doi: 10.3390/cells8121646

Posa F, Grab AL, Martin V, Hose D, Seckinger A, Mori G, i sur.. Copresentation of BMP-6 and RGD ligands enhances cell adhesion and BMP-mediated signaling. Cells [Internet]. 15.12.2019. [pristupljeno 15.05.2024.];8(12). doi: 10.3390/cells8121646

F. Posa, et al., "Copresentation of BMP-6 and RGD ligands enhances cell adhesion and BMP-mediated signaling", Cells, vol. 8, br. 12, Prosinac 2019. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:139774. [Citirano: 15.05.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:105:139774
Podaci o radu
Naslov (engleski)Copresentation of BMP-6 and RGD ligands enhances cell adhesion and BMP-mediated signaling
AutorFrancesca Posa
AutorAnna Luise Grab
AutorVolker Martin
AutorDirk Hose
AutorAnja Seckinger
AutorGiorgio Mori
AutorSlobodan Vukičević
AutorElisabetta Ada Cavalcanti-Adam
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
We report on the covalent immobilization of bone morphogenetic protein 6 (BMP-6) and its co-presentation with integrin ligands on a nanopatterned platform to study cell adhesion and signaling responses which regulate the transdifferentiation of myoblasts into osteogenic cells. To immobilize BMP-6, the heterobifunctional linker MU-NHS is coupled to amine residues of the growth factor; this prevents its internalization while ensuring that its biological activity is maintained. Additionally, to allow cells to adhere to such platform and study signaling events arising from the contact to the surface, we used click-chemistry to immobilize cyclic-RGD carrying an azido group reacting with PEG-alkyne spacers via copper-catalyzed 1,3-dipolar cycloaddition. We show that the copresentation of BMP-6 and RGD favors focal adhesion formation and promotes Smad 1/5/8 phosphorylation. When presented in low amounts, BMP-6 added to culture media of cells adhering to the RGD ligands is less effective than BMP-6 immobilized on the surfaces in inducing Smad complex activation and in inhibiting myotube formation. Our results suggest that a local control of ligand density and cell signaling is crucial for modulating cell response.
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaCells
Brojčani podacivol. 8, br. 12
p-ISSN2073-4409
DOIhttps://doi.org/10.3390/cells8121646
URN:NBNurn:nbn:hr:105:139774
Datum objave publikacije2019-12-15
URL dokumentahttp://doi.org/10.3390/cells8121646
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
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Datum i vrijeme pohrane2024-03-28 10:36:24