Introduction: COVID-19 pandemic has changed our everyday life and impacted the family medicine practice workload. The number of patients per one family medicine practice in Zagreb significantly varies with its location which can limit the quality of healthcare in particular parts of the city. Aim: To determine whether there are changes in the family medicine practice management during five working days of the week depending on the work shift in administrative work, check-ups, consultations, and other diagnostic procedures during the COVID-19 pandemic compared to 2019. Methods: In this cross-sectional study 65 family medicine practices from the western part of Zagreb (45 from Health centre Zagreb - West and 21 private practices) were analysed by monthly work reports for March and April of 2019 and 2021. All noted diagnostic-therapeutic procedures are divided into four main groups (administration, telephone, and e-mail consultations, check-ups and live consultations, and other diagnostic procedures) and analysed depending on the day of the week, shift, and neighbourhood. Results: The amount of administrative work as well as the number of check-ups and live consultations dropped by 5.43 % and 29.17 % respectively, whereas the number of telephone and e-mail consultations increased by 114.98 %, just as the total number of patients increased per day by 8.38 % in the analysed period. The amount of total work done by an average family medicine team depended on the location, 25 % of it was done on Monday and was increased by 10.68 % in 2021 compared with 2019. The ratio of live check-ups in the number of all consultations was decreased by 29 to 58 % depending on the neighbourhood where the practice was located. Three-quarters of family medicine teams did home visits (median 2.5 visits monthly), and 61.53 % of all analysed teams performed ECG (median 7 in practices in Health centre vs. 20 ECGs in private practices per month, p < 0.01). Conclusion: The family medicine practice management was significantly changed in the COVID-19 pandemic which was demonstrated by a double number of telephone and e-mail consultations whereas the number of live check-ups was decreased by one-third. Workload also depended on the family medicine practice location in Zagreb, working shift, and the day of the week.