Objectives: The aim of this paper was to test the characteristics of platelet concentrates produced by plateletpheresis with reduced number of white blood cells in additive solution and subjected to the pathogen inactivation method. Study design: A cross-sectional study with historical data. Materials and methods: Data of the Clinical Institute for Transfusion Medicine in Osijek in the period from 1 November 2019 to 15 March 2020. Data relate to the number of plateletpheresis, the amount of the platelet preparations subjected to the pathogen inactivation method with the use of amotosalen, indications for the application and side-effects of the transfusion therapy. Results: Out of 436 voluntary blood donors, 8 of them were refused as they did not satisfy all requirements. Out of 428 donations made, 404 are compliant (92.7 %). The requests that need to be satisfied by blood preparations before being subjected to the inactivation method with the use of amotosalen were not satisfied by 24 blood preparations. An overall of 71 blood preparations of platelets with reduced number of white blood cells was produced by plateletpheresis and subjected to the pathogen inactivation method, supplied to hospital departments. Most blood preparations were supplied to haematology, cardiovascular and oncology departments, and intensive care units. Conclusion: Transfusion medicine has already gained an enviable level of security with existing procedures before the transfusion therapy was introduced. As we are constantly facing new challenges, viruses, bacteria, and other pathogens, it was necessary to introduce an additional measure for the prevention of undesirable side-effects. The Clinical Institute for Transfusion Medicine in Osijek is the first one in the Republic of Croatia to produce a blood preparation of platelets produced by plateletpheresis with a reduced number of white blood cells in an additive solutions and subjected to the pathogen inactivation method, thus acquiring the highest level of security of transfusion therapy.