Objective: The aims of this study are to determine whether there are differences in opinions of anorexia nervosa, risk factors, early detection and treatment possibilities, character traits of a sufferer between second semester and sixth semester undergraduate nursing students, as well as differences between those that know an anorexia nervosa sufferer personally and those that have never met such a person. Organisation of the study: The study is structured as a cross-sectional study. Subjects and methods: 85 subjects, of which 27 subjects were second semester students and 58 were sixth semester undergraduate nursing students in the academic year of 2016/2017, were subjected to this study. The study was conducted on the premises of Medicinski fakultet Osijek. The chosen method was a questionnaire comprised of questions regarding the subject's age, sex and attending semester, nine multiple choice questions regarding knowledge concerning anorexia nervosa, one question regarding whether the subject knows a person suffering from anorexia nervosa personally, and ten claims which the subject graded on a Likert's scale of 1-5. These claims referred to stereotypical and generalized character traits of an anorexia nervosa sufferer as well as ones which referred to the subject's behaviour in previous or imaginary interaction with an anorexia nervosa sufferer. Results: Nine questions regarding knowledge had the average accuracy of 66,67 %. 9 subjects claimed to know an anorexia nervosa sufferer. Significant statistical differences regarding knowledge of anorexia nervosa were not found, whether based on semester attendancy or knowing a sufferer or not. Significant statistical differences weren't found either in opinions of anorexia nervosa sufferers, based on semester attendancy, or whether the subjects know a sufferer personally or not. Conclusion: If these students were about to work in contact with an anorexia nervosa sufferer once they finish college, they will have to undergo additional education since they do not possess adequate knowledge regarding the disease and sufferer's characteristics. The subjects showed prejudice-free opinions.