Objective: The aim of this research is to determine whether there is a correlation between HIV positive persons with sex, HIV infection and to investigate the risk groups and risk factors in HIV positive respondents. Participants and Methods: Respondents in this study are persons registered in the Center for Voluntary Counseling and Testing of the Public Health Institute of Osijek-Baranja County (Zavod za javno zdravstvo Osječko-baranjske županije – ZZJZOBŽ) from 2005 to 2018. ELISA and CMIA serological methods were been used to test the blood of anonymous volunteers. They are sensitive and specific methods of detecting HIV infection, except during the first few weeks after the infection. Tests may sometimes give false positive results. Because of that each positive finding must be confirmed by a more specific test such as Western blot Results: 1914 persons were tested (1313 M, 601 F). There were 712 more male respondents, which is statistically significant (χ2=264.86; p<0001). There was a total of 37 HIV positive persons, 34 of whom were male, which is statistically significant (χ2 =9.32, p<0.001). In HIV positive men, multiple parameters are statistically significant with p<0.005; (Residency, Not having been previously tested for HIV, Not promiscuous, Not having sex without protection, Not staying abroad for a long period of time). ). Statistical significance has not been established in homosexual contact, which is one of the most commonly known risk factors of HIV positive male subjects. Conclusion: In the Center for voluntary counseling and testing of the Public Health Institute of Osijek-Baranja County, several males with an arithmetic mean age of 33.7 were tested with a standard deviation of 2.5. There was no statistically significant association between HIV positive male and homosexual sexual orientation. There is a statistically significant number of HIV positive persons who had sex without protection.