Objective: The aim of the research was to establish the frequency of usage as well as the variability of immunochromatographic POC tests for determining the causes of respiratory and gastrointestinal infections at the Department of Clinical Microbiology and Parasitology of the University Hospital of Split from 1.1.2020. until 31.12.2020. Material and methods: The basis for carrying out this retrospective research was the data on the usage of POC immunochromatographic tests in infectious disease diagnostics (Biogen, Zagreb, Croatia) obtained from the Department of Clinical Microbiology and Parasitology at the University Hospital of Split. The data processed include both respiratory and gastrointestinal infectious diseases, which were caused by bacteria and viruses. The pathogens of respiratory infections included in the testing were respiratory adenoviruses, RSV, S. pyogenes, influenzas type A and B and SARS-CoV-2. The samples used for testing were nasopharyngeal aspirates, nasopharyngeal swabs, pharyngeal swabs and nasopharyngeal lavages. The pathogens of gastrointestinal infections included were intestinal adenoviruses, H. pylori, norovirus, rotavirus and C. difficile. The sample used for testing was a stool sample of the patients. The data was processed with the Microsoft Office software family (Microsoft, Redmond, WA). Results: Total of 1745 POC immunochromatographic tests for determining the causes of respiratory infections were done. Most of the tests were performed for influenza A and B antigens (554 in total), and the smallest number of tests (31) was done to prove SARS-CoV-2 antigens. The majority of positive results was for the SARS-CoV-2 antigen (29,03%), while the smallest number of them was for the respiratory adenovirus antigens (1,85%). A difference in the share of positives results in relation to the sample used was also noted: for adenoviruses in the nasopharyngeal aspirates (18,18%) in comparison to the nasopharyngeal swabs (0,41%), RSV in the nasopharyngeal aspirates (9,09%) in comparison to the nasopharyngeal swabs (21,95%). The total number of tests performed for the gastrointestinal pathogens in 2020 was 6607. The majority of the positive results was for the toxigenic C. difficile (24,51%) and the smallest number was for intestinal adenoviruses (1,57%). Most of the tests were done for C. difficile, however, approximately the same amount was done on other pathogens. Conclusion: In 2020 at the University Hospital of Split most of the POC immunochromatographic tests were done to determine the cause of gastrointestinal infections. The majority of them were performed for the toxigenic C. difficile. The smallest number was performed for SARS-CoV-2 and S. pyogenes. The biggest share of positive results was noticed for SARS-CoV-2 and C. difficile, while the smallest number was for respiratory and intestinal adenovirusues.