According to the World Health Organization, there were 114,690 solid organ transplantations in 2012 worldwide. There was an increase of 1.8% compared to 2011, which is ≤ 10 % of global needs. Of the total number of transplantations most transplantations are kidney transplantations 42.3% and liver transplantations 18.2%. During 2012, 7893 organs were transplanted in Europe, while the total number of patients in Europe who are waiting for organ transplantation is 15 027. In 2012, Croatia has recorded 400 organ transplantations, while 224 patients are on the waiting list. In Croatia the number of deceased organ donors per million people (pmp) is 34.3, which puts Croatia at the very top in the number of organ donations worldwide. Both in Croatia and in the world there is a permanent increase in the number of people waiting for organ transplantation, which poses a problem for healthcare organizations. The sample in this study is defined by the total number of health care professionals in the University Hospital Center Split, Croatia (KBC Split). Of the total number of health care professionals in the KBC Split, 38% completed the questionnaire. There were 743 survey respondents and the data collected was processed using the methods of descriptive statistics and variance analysis. From the total number of respondents 43% of them are doctors and 38% are nurses/technicians/engineers. The study was conducted with the permission of the Ethics Committee of the KBC Split and the University of Split School of Medicine. Employees of the KBC Split have a very positive attitude towards transplantation and organ explantation (86,3%). Nurses/technicians/engineers have insufficient knowledge of the nature and diagnosis of brain death. The research has shown mistrust of health care professionals in fairness, ethics and legality of the health care system that organizes organ transplantation and explantation in the Republic of Croatia. It is necessary to additionally educate health care professionals about the safety and reliability of the diagnosis of brain death, the nature of brain death, especially health care workers with secondary school diploma and nurses/technicians/engineers. Furthermore, a change in strategy which would make waiting lists transparent and which would inform the families of organ donors about the final destination of donated organs could aid in the goal of gaining trust and thus increase the willingness to participate and promote organ donation.