Objectives: The aim of this study was to investigate the attitudes and practices of students at the University of Split School of Medicine regarding COVID-19 vaccination. Respondents and methods: The participants in this study were students of the University of Split School of Medicine enrolled in the academic year 2022/2023. The attitudes and practices of students regarding COVID-19 vaccination were assessed using a structured questionnaire consisting of four sections. These sections gathered information on sociodemographic characteristics, general and personal opinions about vaccines, perceptions of COVID-19 and the COVID-19 vaccine, personal experiences with COVID-19, and individual behaviors regarding vaccination. We categorized the students into two groups based on their academic year (1st, 2nd, and 3rd-year students as "lower-year students," and 4th, 5th, and 6th-year students as "higher-year students") to compare their attitudes and practices concerning the COVID-19 vaccine and to evaluate the influence of their education on these attitudes and practices. Results: Our study included a total of 181 participants from the University of Split School of Medicine. This cohort consisted of 99 (54.7%) medical students, 51 (28.2%) pharmacy students, and 31 (17.1%) dental medicine students. In general, students exhibited favorable attitudes towards vaccines and vaccination. However, statistically significant differences were found between higher-year and lower-year students. For example, 14.2% of lower-year students agreed with the statement that people receive too many vaccines, compared to 4.9% of higheryear students (p=0.009). Additionally, 46.5% of lower-year students believed that vaccines are important to stay healthy as future healthcare professionals, in contrast to 69.5% of higher-year students (p=0.001). Attitudes towards the COVID-19 vaccine were generally positive but more mixed, with greater skepticism and hesitancy compared to traditional vaccines. Conclusion: Students enrolled at the University of Split School of Medicine generally held positive attitudes towards COVID-19 vaccination. Notably, higher-year students tended to exhibit more favorable attitudes towards COVID-19 vaccination compared to their lower-year counterparts. These findings suggest the potential need to expand the current curriculum to encompass topics related to vaccine hesitancy concerning COVID-19.