Background: The conducted research aims to learn more about the diagnosis and protocol of brain-dead patients at the Neurology Clinic and the Department of Neurological Intensive Care at the University Hospital Center Split. Methods: A retrospective study was conducted at the Neurology Clinic and the Department of Neurological Intensive Care at the University Hospital Center Split, including patients hospitalized from 2015 to 2023 who were diagnosed with brain death. Collected data included the year of admission, the cause of brain death, confirmation of the diagnosis by clinical tests, the ancillary test used to confirm brain death, family consent for organ donation, and the number of patients who donated organs. Results: From 2015 to 2023, 19878 patients were hospitalized at the Neurology Clinic, and 4178 at the Department of Neurological Intensive Care. A total of 102 brain deaths were diagnosed. Statistical analysis found no difference in the number of diagnosed brain deaths during this period (P=0,307). Hemorrhage was the cause of brain death in 69,60% of cases, and ischemia in 25,49%. Statistical analysis did not confirm a difference in the causes of brain death over the years (r=0,25; P=0,51). All cases were initially diagnosed by clinical tests. Most brain deaths were confirmed by evoked potentials (55, 88%). Statistical analysis showed a difference in the use of certain ancillary tests over the period (χ2=41,85; P<0,001). Family consent for organ donation was obtained in 70,58% of the cases. Conclusion: We concluded that there is no significant difference in the number of diagnosed brain deaths at the Neurology Clinic of the University Hospital Center Split between 2015 and 2023. We found that hemorrhages are the most common cause of brain death, and that the most common causes of brain death were equally represented during the period from 2015 to 2023. Statistical analysis revealed a significant change in the use of different ancillary tests for diagnosing brain death between 2015 and 2023. Finally, we determined that during the observed period, there were more organ donors among patients diagnosed with brain death than those who did not donate organs.