Objective. To retrospectively inquire reliability of cytological analysis of pap smear in relation to pathohistological diagnosis of colposcopicaly aimed biopsy and to compare pathohistological results of conus and biopsy of cervix. Design. We have retrospectivelly 106 testees who were operated in the Department of Obstetrics and Gynecology during 2011. We have analyzed following parameters: cytological results of pap smear, pathohistological results of biopsy and pathohistological results of conus. We divided those three parameters into two groups: LSIL and HSIL. Then we compared correlation among pathohistological result of biopsy according to pathohistological result of conus and cytological result of pap smear according to pathohistological result of biopsy and pathohistological result of conus. Settings. Case histories were taken from the archive of the Department of Obstetrics and Gynecology. Pap smear was analyzed at the citological department of the Department of Pathology, Forensic Medicine and Cytology of Clinical Hospital in Split. Pathohistological results of biopsy and conus taken by minor surgery were analyzed at the Department of Pathology, Forensic Medicine and Cytology of Clinical Hospital in Split. Patients and methods. We wrote about examines who underwent surgery of conization or LLETZ. Age, parity, cytological pap smear data, pathohistological results of biopsy, pathohistological results of conus and type of surgery were analyzed. Parameters from cytological results of pap smear were divided into Low-grade Squamous Intraepithelial Lesion (LSIL) that enclose CIN I changes and High-grade Squamous Intraepithelial Lesion (HSIL) that enclose CIN II and CIN III changes. Colposcopy was done preoperatively. Results. We have analyzed results of 106 testees. Comparison of pap smear results and pathohistological results of biopsy showed us that there was statistically significant connection among diagnostical methods (P=0.024). Comparison of pap smear results and pathohistological results of conization showed us that there was no significant connection among observed diagnostical methods (P=0.097). Comparison of pathohistological results of biopsy and patohistologic results of conus showed us that there was statistically significant connection among observed diagnostical methods (P=0.004). Conclusion. With the analysis of citological pap smears we conclude that pap smear is a reliable method in the detection of preneoplastican lesion of cervix. In our pattern we can also be satisfied with reliability of pap smear.