Objectives: Development, optimization and validation of the method for quantitative and qualitative determination of zolmitriptan in tablets by ultra-high performance liquid chromatography coupled to triple quadrupole mass spectrometry. Material and methods: The study used a standard of zolmitriptan from which solutions ranging from 1 ng mL-1 to 1 µg mL-1 were prepared and used to optimize mass spectrometry parameters, liquid chromatography parameters and to validate the method. A standard was also used to determine the appropriate solvent for the samples. Zolmitriptan tablet solutions were used to validate the method and optimize ultrasonic extraction. Results: The solvent of choice for zolmitriptan was a mixture of acetonitrile, ultra-pure water and ammonia in a ratio of 49.5:49.5:1. The mass spectrometer was optimized for a collision energy of 20 eV, a gas temperature of 250 °C and a capillary voltage of 3000 V. Stationary phase with C18 column filler, 0,1 % w/w formic acid as solvent modifier and a mixture of water, acetonitrile and formic acid in the ratio of 800 : 200 : 1 as the mobile phase represent optimized tested chromatographic parameters. Based on the analysis of MS and MS/MS spectra, the structures of the degradation products and the way of their fragmentation were proposed. Ultrasonic extraction is optimized to the duration of 60 minutes. Tested validation parameters including specificity, linearity, accuracy, precision, limit of quantification and detection, stability and robustness resulted with adequate values. Conclusions: Ultra-high performance liquid chromatography coupled to a mass spectrometer is a suitable method for the quantitative and qualitative determination of zolmitriptan in tablets. The method has been validated to ensure its suitability for the determination of zolmitriptan.