In this final thesis, the calculation and dimensioning of the elements of the system for cooling the mixture of mono-ethylene glycol and water to a temperature of -35°C was carried out. In the theoretical part of the work, an introduction to the refrigeration techniques and the use of cooling devices in today's time is given. A technical overview of the basic components of the refrigeration cycle is presented. In the practical part of the work, all the input parameters of the project were defined, based on which the calculation and dimensioning of the system was performed. Based on the calculation of the transmission heat gains of the cooling vessel and the necessary capacity to cool the mixture of mono -ethylene glycol and water to a temperature of -35°C, the calculation and sizing of the evaporator, made of copper pipes, is presented. Thermal expansion valve and condensing unit with compressor are dimensioned and selected. Finally, a conceptual design of the refrigeration system for deep cooling is given.