Through this final paper, the goal is to investigate the importance of entrepreneurial centers in the Republic of Croatia, and entrepreneurial infrastructure in general in relation to entrepreneurship. Entrepreneurship centers are centers for entrepreneurial training and professional and advisory assistance to entrepreneurs. Entrepreneurial centers are developed depending on the number of entrepreneurs in a certain area, that is, on the overall need for the establishment of such centers in a certain area. Entrepreneurial infrastructure in the Republic of Croatia consists of entrepreneurial zones and entrepreneurial support institutions that enable entrepreneurial activities to be carried out under standardized conditions with high infrastructure equipment. The term entrepreneurial support institutions includes development agencies, entrepreneurial centers, business incubators, entrepreneurial accelerators, business parks, science and technology parks, and competence centers. The purpose of the legislative framework is to enable existing and potential users to start and operate under standardized conditions with high infrastructural equipment within entrepreneurial zones and institutions to support entrepreneurship and within the framework of a competitive and transparent incentive system. Entrepreneurship centers in the Republic of Croatia are very important in terms of the training of entrepreneurs as physical persons, but also in terms of entrepreneurship as a process, and in the last case also in terms of the progress of the entire economy, therefore, through this thesis, such importance is shown on the example of the Entrepreneurship Center Split, the Entrepreneurship Center Zovnica Center and the Entrepreneurial Center of the Zagreb County.