Physiological potential of lactic acid bacteria has been insufficiently investigated and implemented in biotechnological industrial production of different products. Few latest investigations indicate robustness of those bacteria as well as their potential applications in manufacturing of value added biochemicals and other products that are remarkably accepted in a market. In this work a capacity of a strain Leuconostoc mesenteroides LMG 7954 to use sucrose, besides glucose, as a source of carbon and energy was explored. Microaerophilic and aerobic conditions of laboratory scale batch cultivations were created and a growth curve of those heterofermentative lactic acid bacterium was followed. Besides, survival of L. mesenteroides LMG 7954 in a reaction mixture containing high concentration of glycerol (c = 2,0 mol/L), another potential source of a carbon for the strain, and sucrose (c = 0,8 mol/L) was characterized. In this reaction mixture with high osmotic pressure bacterial culture with the cells from the middle of exponential growth phase were treated by ultrasound. Duration of growth phases and specific growth rate of the strain L. mesenteroides LMG 7954 were estimated based on the experimental results obtained under selected conditions of batch cultivations. Obtained values represents excellent starting point for further investigations of physiology of the strain and its implementation in sustainable biotechnological production of different products and high added value utilities.