C2C12 cells were originally derived from satellite cells from the thigh muscle of female mouse donor after a crush injury. They are easily cultured and differentiated in cell culture and often are used as a model system for studying muscle growth and differentiation. The aim of this thesis was to study the growth and morphological characteristic of C2C12 cells in proliferation and differentiation media. During the experiment, once the cells reached 60-70 % confluence, a part of cells were transferred to a differentiation medium containing 2% horse serum, while the other part remained to grow in culture medium with 10% foetal bovine serum. The growth characteristics of C2C12 cells were determined by Trypan blue method during 264 h, while morphological changes in C2C12 cells were analysed by inverted light microscope after crystal-violet staining. Obtained results showed that cells cultivated in 2% horse serum achieved a 2.5x lower total cell yield in comparison to the cells cultivated in a proliferation medium supplemented by 10% foetal bovine serum. Additionally, morphological changes in C2C12 cells during the processes of proliferation and differentiation were observed. Cultivation of C2C12 cells in a differentiation medium resulted in cell differentiation and the formation of multinucleated, elongated myotubes. At the same time, cells cultured in a proliferation medium retained their typical undifferentiated, myoblastic morphology.