Climate change and the increase in greenhouse gas emissions emphasize the need for progress and improvement of the ecological sustainability of the food system. The aim of this study was to examine the attitudes of consumers from the Republic of Croatia regarding sustainable diet and whether there are differences of socio-demographic characteristics. In an online survey of 517 respondents, it was determined that among the most important motives for adopting sustainable eating habits are buying local food (n=459), shopping according to real needs (n=385) and reducing the use of plastic (n=288). More than half of the respondents (59.6 %) would not accept cultured meat as a new source of protein. Significantly more men and those living in cities would still accept the consumption of laboratory-grown meat (p˂0.05). Almost 73.1% of respondents answered that they would never and unlikely consume insect flour. For the acceptance of new sources of protein (such as insects), women and those under 30 years of age would consider concerns for health and sustainability and animal welfare to be significantly more important factors (p˂0.05), and taste for younger respondents. This research could be the basis for future research in which changes in the attitudes of Croatian citizens will continue to be investigated.