Exotic fruit by-products represent food waste and are used as raw material for the produc tion of new or enrichment of existing products in the food, pharmaceutical and food supplement indus tries. Avocado and banana by-products contain a high level of various bioactive substances that have a beneficial effect on health, industry and ecology. The aim of this work was to examine the influence of the extraction solvent on the antioxidant activity of avocado and banana peels. In order to evaluate the influence of the extraction solvent, three different deep eutectic solvents with 10 and 30% water were used and their effectiveness was compared with a 30% aqueous ethanol solution as an example of a conventional solvent. Three spectrophotometric methods, DPPH, FRAP and ABTS were used to determine antioxidant activity. Regardless of the method, the most effective extraction solvents were choline chloride and glycerol (1:3) with 30% H₂O and choline chloride and lactic acid (1:3) with 30% H₂O for avocado extracts, and the same for banana extracts. extraction solvents, but with 10% H₂O. When extracting avocado peel, deep eutectic solvents were equally effective as 30% aqueous ethanol solution, while with banana peel ethanol extract had significantly lower antioxidant activity. It can be concluded that deep eutectic solvents have a high application potential in the isolation of components with antioxidant activity from avocado and banana peels.