The aim of this study was to determine the effect of treatment with Rivergreen® on growth and development of endive transplants in controlled environment. Rivergreen® is a new product obtained by milling and tribomechanical activation of the river Drava rock sediment. The research was conducted during 2018 at the Laboratory for Vegetable, Flowers, Medicinal and Spice Herbs at the Faculty of Agrobiotechnical Sciences Osijek. Two varieties of endive, Eskariol green and Eskariol yellow were used in the study, following the growth and development of the endive until the transplants stage. The results of this research have revealed that the influence of Rivergreen® on growth and development of endive depends on cultivar as well as on the observed properties. It was found that Rivergreen® did not have a significant influence on the fresh and dry weight of Eskariol green, while it influenced on the same properties of the Eskariol yellow cultivar significantly increasing aboveground part fresh and dry weight. It was also determined that the number of leaves of Eskariol green was higher influenced by Rivergreen® treatment. The leaf width in the specified cultivar was not affected by Rivergreen® treatment. In the Eskariol yellow variety, Rivergreen® had no effect on the number of leaves, but the width of leaves was higher when treated with Rivergreen®. The leaf length in both varieties was not affected by Rivergreen® treatment. From obtained results, it can be concluded that Rivergreen® in most of the observed properties, has a different influence depending on the cultivar. Further research is needed to determine the true effect of Rivergreen® on final yield and quality of endive.