prikaz prve stranice dokumenta Influence of milk product type and its initial contamination on the efficiency of different methods for detection of Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli O157:H7
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Influence of milk product type and its initial contamination on the efficiency of different methods for detection of Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli O157:H7
Mljekarstvo : časopis za unaprjeđenje proizvodnje i prerade mlijeka, 68 (2018), 1; 3-11. https://doi.org/10.15567/mljekarstvo.2018.0101
Antunović, Boris; Kovaček, Ivančica; Gvozdanović, Kristina; Grčević, Manuela; Gantner, Vesna; Poljak, Vedran; Ostović, Mario; Pavičić, Željko; Ahmetović, Nihada

Institucijski repozitorij: Repozitorij Fakulteta agrobiotehničkih znanosti Osijek

Citirajte ovaj rad

Antunović, B., Kovaček, I., Gvozdanović, K., Grčević, M., Gantner, V., Poljak, V. ... Ahmetović, N. (2018). Influence of milk product type and its initial contamination on the efficiency of different methods for detection of Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli O157:H7. Mljekarstvo : časopis za unaprjeđenje proizvodnje i prerade mlijeka, 68. (1), 3-11. doi: 10.15567/mljekarstvo.2018.0101

Antunović, Boris, et al. "Influence of milk product type and its initial contamination on the efficiency of different methods for detection of Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli O157:H7." Mljekarstvo : časopis za unaprjeđenje proizvodnje i prerade mlijeka, vol. 68, br. 1, 2018, str. 3-11. https://doi.org/10.15567/mljekarstvo.2018.0101

Antunović, Boris, Ivančica Kovaček, Kristina Gvozdanović, Manuela Grčević, Vesna Gantner, Vedran Poljak, Mario Ostović, Željko Pavičić i Nihada Ahmetović. "Influence of milk product type and its initial contamination on the efficiency of different methods for detection of Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli O157:H7." Mljekarstvo : časopis za unaprjeđenje proizvodnje i prerade mlijeka 68, br. 1 (2018): 3-11. https://doi.org/10.15567/mljekarstvo.2018.0101

Antunović, B., et al. (2018) 'Influence of milk product type and its initial contamination on the efficiency of different methods for detection of Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli O157:H7', Mljekarstvo : časopis za unaprjeđenje proizvodnje i prerade mlijeka, 68(1), str. 3-11. doi: 10.15567/mljekarstvo.2018.0101

Antunović B, Kovaček I, Gvozdanović K, Grčević M, Gantner V, Poljak V, i sur.. Influence of milk product type and its initial contamination on the efficiency of different methods for detection of Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli O157:H7. Mljekarstvo : časopis za unaprjeđenje proizvodnje i prerade mlijeka [Internet]. 10.01.2018. [pristupljeno 19.12.2024.];68(1):3-11. doi: 10.15567/mljekarstvo.2018.0101

B. Antunović, et al., "Influence of milk product type and its initial contamination on the efficiency of different methods for detection of Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli O157:H7", Mljekarstvo : časopis za unaprjeđenje proizvodnje i prerade mlijeka, vol. 68, br. 1, str. 3-11, Siječanj 2018. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:151:091889. [Citirano: 19.12.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:151:091889
Podaci o radu
Naslov (engleski)Influence of milk product type and its initial contamination on the efficiency of different methods for detection of Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli O157:H7
AutorBoris Antunović
AutorIvančica Kovaček
AutorKristina Gvozdanović
AutorManuela Grčević
AutorVesna Gantner
AutorVedran Poljak
AutorMario Ostović
AutorŽeljko Pavičić
AutorNihada Ahmetović
Autorova ustanovaSveučilište Josipa Jurja Strossmayera u Osijeku
Fakultet agrobiotehničkih znanosti Osijek
(Zavod za animalnu proizvodnju i biotehnologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOTEHNIČKE ZNANOSTI
Poljoprivreda (agronomija)
Stočarstvo
Sažetak (engleski)
This paper investigates differences in efficacy of isolating pathogenic bacteria Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli O157:H7 between conventional cultivation (ISO method) and immunomagnetic separation (IMS method) related to the types of dairy products and initial numbers of bacteria. Different milk products (dairy pudding- vanilla or chocolate; a mixture of yoghurt and pudding; solid, liquid and fruit yoghurt; AB culture - with or without fruit; cheese spread) were intentionally contaminated with different numbers (≈10 and ≈30) of live cells of the observed bacteria per mL. The obtained results showed that the classical ISO procedure still represents an equally adequate method for the detection of S. Enteritidis and L. monocytogenes in dairy products as well as the IMS method. However, the ISO method was found to be inefficient for determination of E. coli O157:H7 when the initial contamination was low (≈10 live cells per mL). In such cases, even the IMS method appeared to be inefficient when used for fermented dairy product analysis. Fermented dairy products in contrast to the non-fermented ones, still represent a challenge for the development of routine detection methods, especially for S. Enteritidis, whilst the detection of L. monocytogenes and E. coli O157:H7 has improved by introducing the IMS method. The largest difference in the ability to detect bacteria in dairy product samples with reference to the initial number of bacteria by both methods was in the detection of E. coli O157:H7. The choice of broth (non-selective fluid broth vs. selective fluid broth) did not matter in the in the detection of S. Enteritidis and L. monocytogenes by applying the IMS procedure. However, for the detection of E. coli O157:H7 the application of modified tripton-soya broth with novobiocin (mTSB+Nb) has proved to be superior when compared to using the buffered peptone water (BPW). The presented results may be of importance as a scientific basis for future determination of standard methods related to laboratory detection of pathogens in dairy products.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - domaća recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaMljekarstvo : časopis za unaprjeđenje proizvodnje i prerade mlijeka
Brojčani podacivol. 68, br. 1, str. 3-11
p-ISSN0026-704X
e-ISSN1846-4025
DOIhttps://doi.org/10.15567/mljekarstvo.2018.0101
URN:NBNurn:nbn:hr:151:091889
Datum objave publikacije2018-01-10
URL dokumentahttps://hrcak.srce.hr/192294
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2024-05-30 22:12:41