prikaz prve stranice dokumenta Aprotinin interacts with substrate-binding site of human dipeptidyl peptidase III
  Preuzmi
PDF 3.71 MB
Znanstveni rad - Izvorni znanstveni rad
Aprotinin interacts with substrate-binding site of human dipeptidyl peptidase III
JOURNAL OF BIOMOLECULAR STRUCTURE AND DYNAMICS, 37 (2018), 14; 3556-3606. https://doi.org/10.1080/07391102.2018.1521343
Agić, Dejan; Brkić, H.; Kazazić, S.; Tomić, A.; Abramović, M.

Institucijski repozitorij: Repozitorij Fakulteta agrobiotehničkih znanosti Osijek

Citirajte ovaj rad

Agić, D., Brkić, H., Kazazić, S., Tomić, A. i Abramović, M.. Aprotinin interacts with substrate-binding site of human dipeptidyl peptidase III. JOURNAL OF BIOMOLECULAR STRUCTURE AND DYNAMICS, 37. (14), 3556-3606. doi: 10.1080/07391102.2018.1521343

Agić, Dejan, et al. "Aprotinin interacts with substrate-binding site of human dipeptidyl peptidase III." JOURNAL OF BIOMOLECULAR STRUCTURE AND DYNAMICS, vol. 37, br. 14, str. 3556-3606. https://doi.org/10.1080/07391102.2018.1521343

Agić, Dejan, H. Brkić, S. Kazazić, A. Tomić i M. Abramović. "Aprotinin interacts with substrate-binding site of human dipeptidyl peptidase III." JOURNAL OF BIOMOLECULAR STRUCTURE AND DYNAMICS 37, br. 14 3556-3606. https://doi.org/10.1080/07391102.2018.1521343

Agić, D., et al. 'Aprotinin interacts with substrate-binding site of human dipeptidyl peptidase III', JOURNAL OF BIOMOLECULAR STRUCTURE AND DYNAMICS, 37(14), str. 3556-3606. doi: 10.1080/07391102.2018.1521343

Agić D, Brkić H, Kazazić S, Tomić A, Abramović M. Aprotinin interacts with substrate-binding site of human dipeptidyl peptidase III. JOURNAL OF BIOMOLECULAR STRUCTURE AND DYNAMICS [Internet]. [pristupljeno 26.11.2024.];37(14):3556-3606. doi: 10.1080/07391102.2018.1521343

D. Agić, H. Brkić, S. Kazazić, A. Tomić i M. Abramović, "Aprotinin interacts with substrate-binding site of human dipeptidyl peptidase III", JOURNAL OF BIOMOLECULAR STRUCTURE AND DYNAMICS, vol. 37, br. 14, str. 3556-3606. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:151:779701. [Citirano: 26.11.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:151:779701
Podaci o radu
Naslov (engleski)Aprotinin interacts with substrate-binding site of human dipeptidyl peptidase III
AutorDejan Agić
AutorH. Brkić
AutorS. Kazazić
AutorA. Tomić
AutorM. Abramović
Autorova ustanovaSveučilište Josipa Jurja Strossmayera u Osijeku
Fakultet agrobiotehničkih znanosti Osijek
(Zavod za agroekologiju i zaštitu okoliša)
Znanstveno / umjetničko
područje, polje i grana		BIOTEHNIČKE ZNANOSTI
Poljoprivreda (agronomija)
Agrokemija
Sažetak (engleski)
Human dipeptidyl peptidase III (hDPP III) is a zinc-exopeptidase of the family M49 involved in final steps of intracellular protein degradation and in cytoprotective pathway Keap1-Nrf2. Biochemical and structural properties of this enzyme have been extensively investigated, but the knowledge on its contacts with other proteins is scarce. Previously, polypeptide aprotinin was shown to be a competitive inhibitor of hDPP III hydrolytic activity. In this study, aprotinin was first investigated as a potential substrate of hDPP III, but no degradation products were demonstrated by MALDI-TOF mass spectrometry. Subsequently, molecular details of the protein–protein interaction between aprotinin and hDPP III were studied by molecular modeling. Docking and long molecular dynamics (MD) simulations have shown that aprotinin interacts by its canonical binding epitope with the substrate binding cleft of hDPP III. Thereby, free N-terminus of aprotinin is distant from the active-site zinc. Enzyme-inhibitor complex is stabilized by intermolecular hydrogen bonding network, electrostatic and hydrophobic interactions which mostly involve constituent amino acid residues of the hDPP III substrate binding subsites S1, S1’, S2, S2’ and S3’. This is the first study that gives insight into aprotinin binding to a metallopeptidase.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - domaća recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaJOURNAL OF BIOMOLECULAR STRUCTURE AND DYNAMICS
Brojčani podacivol. 37, br. 14, str. 3556-3606
p-ISSN0739-1102
e-ISSN1538-0254
DOIhttps://doi.org/10.1080/07391102.2018.1521343
URN:NBNurn:nbn:hr:151:779701
Datum prihvaćanja za tisak2018-09-03
URL dokumentahttps://www.tandfonline.com/doi/pdf/10.1080/07391102.2018.1521343?needAccess=true
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2024-06-18 08:27:02