A study aimed at investigating the possibilities of micropropagation of rosemary (Rosmarinus officinalis L.) in in vitro plant tissue culture conditions was conducted at the Faculty of Agrobiotechnical Sciences Osijek during 2023. The research utilized MS nutrient medium with and without the addition of hormones BAP and IBA. The study examined the differences in productivity and morphological indicators between two types of explants (single shoots and basal type). After 30 days of culture, all treatments developed ideal biomass necessary for the evaluation of the research. Statistical analysis showed significant differences between the types of explants in the number of shoots and multiplication, while there were no significant differences in the number of lateral shoots, callus mass, and vegetative mass. The control treatment and the treatment with hormones on the basal type of explants resulted in the highest number of shoots. The highest number of lateral shoots was recorded in the hormone treatment that included single shoots type explants. The length of shoots and the number of leaves were greater in treatments with single shoots explants compared to basal explants. The highest productivity (multiplication) was achieved in the control treatment with basal type explants. The MS nutrient medium without the addition of plant hormones and the basal type explants represent valuable guidelines for further research in the development of protocols for the production of rosemary biomass, planting material, or in vitro multiplication. Further research should focus on examining the effects of higher concentrations of hormones and the application of other cytokinin and auxins, including meta- Topoline (mT), which is currently very relevant in research practice.