Investigation of maize reaction on nitrogen fertilization under conservation soil tillage were carried out during the 2012/2013 year on hydromorphic soil types on two locations: Čačinci (GPS: Long. 17.86336 E; Lat. 45.61316 N) and near Magadenovci (GPS: Long. 18.70648 E; Lat. 45.55555 N). The field experiments included three levels of nitrogen fertilization on three different conservation soil tillage treatments. N1 level of fertilization was 30% lower compared to fertilization recommendation, N2 level of fertilization was equal to fertilizer recommendation and N3 level of fertilization was 30% higher compared to fertilization recommendation. Conservation soil tillage systems was of: PO- subsoiling, RA- chiselling and DS- direct seeding. According to the data, the level of nitrogen fertilization and specific locations were significantly impacted almost all tested parameters (plant height, plant weight, weight of cob, grain weight per cob, biological and agricultural yield). Conservation tillage treatment had a significant effect on all parameters except at the height of the plants, hectoliter weight and mass of 1000 grains. There were not found any statistically significant impacts of treatments on hectoliter weight and mass of 1000 grains. The largest agricultural and biological maize yields were achieved on location near Magadenovci (biological yield was 49.1 t ha-1 and agricultural yield was 12.8 t ha-1), on subsoiling soil tillage treatments (biological yield was 45.1 t ha-1 and agricultural yield was 12.1 t ha-1) and on the fertilization treatment according to recommendation (biological yield was 43.7 t ha-1 and agricultural yield was 11.6 t ha-1).