prikaz prve stranice dokumenta Ecto-ADPase activity in the rat renal brush-border membranes
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Ecto-ADPase activity in the rat renal brush-border membranes
Croatica Chemica Acta, 68 (1995), 3; 491-510. urn:nbn:hr:163:516925
Žanić-Grubišić, Tihana; Griparić, Lorena; Zrinski, Renata; Floegel, Mirna

Institucijski repozitorij: Repozitorij Farmaceutsko-biokemijskog fakulteta Sveučilišta u Zagrebu

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Žanić Grubišić, T., Griparić, L., Zrinski, R. i Floegel, M. (1995). Ecto-ADPase activity in the rat renal brush-border membranes. Croatica Chemica Acta, 68. (3), 491-510. Preuzeto s https://urn.nsk.hr/urn:nbn:hr:163:516925

Žanić Grubišić, Tihana, et al. "Ecto-ADPase activity in the rat renal brush-border membranes." Croatica Chemica Acta, vol. 68, br. 3, 1995, str. 491-510. https://urn.nsk.hr/urn:nbn:hr:163:516925

Žanić Grubišić, Tihana, Lorena Griparić, Renata Zrinski i Mirna Floegel. "Ecto-ADPase activity in the rat renal brush-border membranes." Croatica Chemica Acta 68, br. 3 (1995): 491-510. https://urn.nsk.hr/urn:nbn:hr:163:516925

Žanić Grubišić, T., et al. (1995) 'Ecto-ADPase activity in the rat renal brush-border membranes', Croatica Chemica Acta, 68(3), str. 491-510. Preuzeto s: https://urn.nsk.hr/urn:nbn:hr:163:516925 (Datum pristupa: 28.12.2024.)

Žanić Grubišić T, Griparić L, Zrinski R, Floegel M. Ecto-ADPase activity in the rat renal brush-border membranes. Croatica Chemica Acta [Internet]. 1995. [pristupljeno 28.12.2024.];68(3):491-510. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:163:516925

T. Žanić Grubišić, L. Griparić, R. Zrinski i M. Floegel, "Ecto-ADPase activity in the rat renal brush-border membranes", Croatica Chemica Acta, vol. 68, br. 3, str. 491-510, 1995. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:163:516925. [Citirano: 28.12.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:163:516925
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Naslov (engleski)Ecto-ADPase activity in the rat renal brush-border membranes
AutorTihana Žanić-Grubišić
AutorLorena Griparić
AutorRenata Zrinski
AutorMirna Floegel
Autorova ustanovaSveučilište u Zagrebu
Farmaceutsko-biokemijski fakultet
(Zavod za medicinsku biokemiju i hematologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Farmacija
Medicinska biokemija
Sažetak (engleski)
Brush-border membrane vesicles purified from rat kidney cortex exhibit an ectoenzyme activity responsible for the hydrolysis of both ATP and ADP, as well as of other nucleoside tri- and diphosphates. In the presence of Ca2+ ions, ADP hydrolysis follows the simple Michaelis-Menten kinetics assuming a single catalytic site. The real substrate for ADPase is a divalent cation conjugated ADP. The pH optimum for the hydrolysis is between 7.2 and 8.6. ADP and ATP hydrolysis show similar heat denaturation curves, and are both resistant to limited proteolysis and to inhibitors of other known ATPases. The enzyme activity is inhibited by: diethyl py- rocarbonate, dithiothreitol, high concentrations of both IV-ethylmale- imide and azide. The diethyl pyrocarbonate inhibition could be reversed by hydroxylamine, indicating the involvement of histidine and/or tyrosine residues in the reaction. It is proposed that both ADPase and ATPase activities reside within the same enzyme protein.
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaCroatica Chemica Acta
Brojčani podacivol. 68, br. 3, str. 491-510
p-ISSN0011-1643
e-ISSN1334-417X
URN:NBNurn:nbn:hr:163:516925
Datum objave publikacije1995
URL dokumentahttps://hrcak.srce.hr/136710
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2020-04-29 14:59:53