Endothelial nitric oxide synthase (eNOS) is an enzyme of exceptional importance for the vascular system. It produces nitric oxide (NO), which has a vasodilating effect in the vascular endothelium, contributes to the regulation of blood pressure, inhibits platelet aggregation and their adhesion to the blood vessel wall. Endothelial NO synthase shows a protective effect after cerebral ischemia because it produces NO that promotes vasodilation and inhibits microvascular aggregation and adhesion. The eNOS -786 T > C and 894 G > T polymorphisms lead to reduced NO production or increased degradation of the mature enzyme, so individuals carrying these polymorphisms are potentially at higher risk of adverse vascular events. The aim of this study was to investigate the relationship between eNOS polymorphisms and stroke in children. Strokes in children, although rare, are on the rise today. They are serious conditions that, in most cases, leave a severe neurological deficit. In this study, an association was found between the eNOS -786 T > C polymorphism and CSVT in children according to the codominant (p = 0,026) and overdominant (p = 0,007) inheritance model. The association of the eNOS 894 G > T polymorphism and CSVT in female children according to the codominant (p = 0,031), dominant (p = 0,025) and overdominant (p = 0,019) inheritance models was also found. This study was performed on a small group of patients and the results need to be verified by more extensive studies.