Considering the high incidence of breast cancer that is present in our population and the development of various treatment options for this disease, it is of great importance to develope new analytical methods for therapeutic drug monitoring of the drugs of interest. The aim of this study was to develop a fast and simple capillary electrophoretic method for the analysis of anastrozole, which will also be more environmentally friendly and cheaper than the already respected HPLC method. Micellar electrokinetic chromatography was selected as a suitable technique. This analysis was performed on a 35 cm long capillary of fused silica, with effective length of 27 cm, and an inner diameter of 50 μm. The samples were hydrodynamically injected into the capillary under 50 mbar pressure, at a 20 °C temperature for 4 seconds. A wavelength of 210 nm was used to detect anastrozole. The effects of borate buffer (15 – 45 mM) and SDS (15 – 50 mM) concentration, as well as temperature (17.5 – 27.5 °C) and voltage (22.5 – 30 kV) on the migration time, peak area, and theoretical plates were observed. Considering the experimental results, 20 mM borate buffer, 45 mM SDS, and a temperature of 20 ° C were taken as optimal values. It is required to further optimize this method using a lower concentration of anastrozole spiked in plasma. The subject of further research will also be the optimization of conditions in simultaneous analysis of anastrozole and other drugs that are used as a combination therapy for treatment of advanced hormone-dependent HER2 - negative breast cancer: palbocyclib, ribocyclib or abemacyclib.