Centrifugal partition chromatography (CPC) is a recent preparative technique that is increasingly used in isolation, purification and concentration of natural products. In this thesis, the theoretical finding about this type of liquid chromatography without a solid support and the possibilities of its application in the field of natural products are presented. Since extracts obtained from plant raw material are quite complex mixtures, the separation of their bioactive components is a complex process. The CPC method combines extraction and chromatography principles and it can be used to isolate substances with a wide range of polarity. The characteristics of this system are liquid stationary and mobile phases, so analysis and separation of the components of natural products are based on the distribution of solutes between two immiscible liquid phases. The most commonly used two phase solvent system for this purpose is hexane / ethyl acetate / methanol / water. The technique has proven to be an effective alternative to conventional preparative chromatographic techniques and it is characterized by a shorter purification time and possibility of injecting a larger quantity of samples, as well as by obtaining a higher quantity of high purity compounds (> 90 %). Scientific researches prove the success of application in the isolation and purification of various group of natural compounds such as cannabinoids, lignans, coumarins, anthocyanins, alkaloids, flavonoids, stilbenes, xanthones, monoterpens, sesquiterpens, saponins and various pigments. Given the efficiency of preparative purification, this type of chromatography could satisfy the demand for large quantities of pure natural compounds necessary for further biological, pharmacological and clinical research, of for the production of standards for the purposes of chemical analyses or for the production of fractionated and concentrated natural products of interest for pharmaceutical, food and cosmetic industry.