Introduction: BMP1-3 achieves a profibrotic effect through increased ECM expression and deposition, which can result in an aesthetically visible scar. The profibrotic effect of BMP1-3 is thought to be partly caused by the combined decrease in decorin expression and increased expression of Col1A1 and TGFβ. This research explores the effect of polyclonal antibodies developed on BMP1-3 on the expression of Col1A1, TGFβ-1 and decorin in scar tissue in vivo using different concentrations and methods of antibody application in an experimental model using rats. Methods: After rat treatment with different doses and methods of application of BMP1-3 polyAb and BMP1-3, RNA was isolated from the obtained skin tissue homogenates and analysis of gene expression of Col1A1, TGFβ and decorin was performed by real-time polymerase chain reaction. Results: Administration of BMP1-3 polyAb subcutaneously at three concentration levels did not result in different gene in expression between treated and control side of body in each group of animals, as well as between groups treated with different antibody doses. For the groups treated with BMP1-3 polyAb intravenously and BMP1-3 protein subcutaneously, gene expression in the treated group was not statistically significantly changed when compared to the control group. Conclusion: It is possible that BMP1-3 polyAb shows a more significant effect on posttranslational modifications instead to the transcription of collagen, TGFβ and decorin genes. Despite the lack of a difference in expression between the treated and control groups , it is necessary to take into account the results obtained by future histological analysis, and interpret the potential importance of BMP1-3 polyAb to reduce skin scar size in rats accordingly.