In a medical-biochemical laboratory, each analytical device must undergo a verification or validation procedure before being put into routine operation, which assesses its applicability for the intended measurement procedure. Most often, the analytical characteristics of the device that the manufacturer achieved during factory validation, precision, accuracy, measuring range, but also comparison with a possibly previously used system are verified in order to assess the impact of changing the analytical system on the clinical decision. With hemoglobin electrophoresis, it is possible to detect qualitative and quantitative disorders in the synthesis of globin chains, and nowadays in clinical laboratories diagnostics of hemoglobinopathy is most often carried out using the automated capillary zone electrophoresis. The aim of this study was to evaluate analytical performance of the miniCAP flex piercing system before putting it into routine operation. The system uses two capillaries, in which hemoglobin variants are separated for 8 minutes at 9600V and pH 6.8. In healthy adult subjects, hemoglobin consists of HbA and HbA2, while in newborns we also find HbF. Evaluation was performed according to the CLSI EP15-A3 document using three different control samples that included different hemoglobin variants (HbA, HbF, HbS, HbC and HbA2). The achieved results for precision and accuracy are in accordance with the manufacturer's specifications. Comparison of the results with the system currently used in routine work, Capillarys 2, indicated excellent correlation between results. A smaller constant deviation in HbF values can be explained by the wide range of values covered by this evaluation. Evaluation results show that the tested analytical system for capillary electrophoresis of hemoglobin, miniCAP flex piercing meets the set acceptance criteria and can be used in routine laboratory work. As an additional value of using this device, the possibility of conducting analysis from a blocked primary test tube was recognized, which improved the safety of technician in laboratory conditions.