Beta carotene, lycopene and curcumin are plant pigments that show great antioxidant potential. They are important in maintaining human health and preventing diseases. Due to their antioxidant effect, they are considered nutraceuticals - valuable components of new formulas of enteral nutrition. Enteral nutrition, due to its role of supplementing human nutrition in conditions and diseases that compromise ingestion through a healthy diet, is rich in macronutrients and micronutrients. Dietary fibers are an essential component that ensures healthy digestion and can have a probiotic effect, but due to its physical and chemical properties, its interaction with carotenoids and curcumin can reduce their bioavailability. The stability of beta carotene, lycopene, and curcumin in simulated digestive conditions and their bioavailability as affected by different dietary fibers mixtures were examined in this master thesis. Beta carotene and lycopene showed stability during the simulation of digestion conditions, while the concentration of curcumin decreased significantly (to about 70%). The bioavailability of lycopene was reduced by all 10 dietary fiber mixtures that were analyzed, while the bioavailability of beta-carotene and curcumin remained unchanged when analyzed with some of 10 mixtures. Two standard mixtures STAND-2 and STAND-3 have a significant effect on reducing the bioavailability of all three analyzed compounds. Innovative mixtures have different effects on compounds, depending on their composition. All innovative mixtures have little effect on the bioavailability of beta-carotene. The highest bioavailability of lycopene was shown with the mixture INOV-1B (98.3%). The highest bioavailability of curcumin was shown with the STAND-3 mixture (113.7%). With obtained results it can be concluded there is similarity between two standard mixtures (STAND-2 and STAND-3), and there is difference between composition of the other 8 mixtures with different affinity towards the binding of analyzed compounds.