Objective The aim of this expert thesis is to describe the properties of bispecific antibodies approved for clinical use and those in clinical trials, to analyse the methods of their production and to provide insight into the therapeutic possibilities of such antibodies. Materials and methods The thesis provides an overview of the development of bispecific antibodies and their therapeutic applications. The review of the scientific literature describes in more detail the methods for the correct linking of light and heavy chains. The thesis describes the approved bispecific antibodies and their therapeutic use, as well as those that are in different stages of development. Results Bispecific antibodies combine two or more antigen-recognizing elements into a single molecule with the ability to link to two or more targets. By acting on multiple targets simultaneously, the effectiveness of the therapy increases and the need for frequent drug administration is reduced. In order to link different heavy and light chains into one molecule, new methods have been developed. Some of these methods are quadroma technology, buttonhole technique, common light chain, CrossMAb technology, SEED, WuxyBody, etc. Thanks to these methods, today there are seven bispecific antibodies approved for clinical use; in addition to these, there are more than 180 bispecific antibodies approved in preclinical trials, as well as more than 50 in different stages of clinical trials. Conclusion The development of biotechnology and genetic engineering has facilitated the discovery of new methods for correct linking of light and heavy chains that have enabled the development of functional bispecific antibodies used in clinical practice. Bispecific antibodies have shown significant improvement in efficacy compared to previous therapy. Progress is evident in treatment outcomes, in the time it takes to achieve a therapeutic response, and in the time to disease worsening. In addition to better treatment results, bispecific antibodies have also been shown to be more cost-effective than the previous therapy.