Benzodiazepines are among the most commonly prescribed drug classes, and their consumption is steadily increasing. However, their long-term use is not recommended due to the various side effects they can cause. Consequently, prolonged benzodiazepine use poses a common therapeutic problem. Older individuals are particularly susceptible to the effects of benzodiazepines, but healthcare professionals frequently prescribe this class of drugs to them. Hence, there is a growing need to rationalize therapy and potentially reduce benzodiazepine usage. Deprescribing refers to the process of discontinuing inappropriate medication or reducing the dosage with the goal of achieving the best therapeutic outcome for the patient, under the supervision of healthcare professionals. The objective of this study was to assess the appropriateness of benzodiazepine use in older individuals residing in nursing homes and to identify patients who could benefit from deprescription. This study involved residents of nursing homes in the regions of Dalmatia, Slavonia, and the City of Zagreb, aged 65 years and above. Data was collected using a standardized interRAI questionnaire designed for comprechensive geriatric assessment in long-term care facilities. Based on deprescription guidelines for benzodiazepines, four criteria were defined for the purpose of this research to determine the need for deprescription. The participants' mean age was 82.52 ± 6.223 years, with 40.4% classified as very old (>85 years), and 76.5% were female. The study found that 127 participants were using benzodiazepines in their therapy, and among them, 106 (83.5%) would be potential candidates for deprescription. The most frequently identified reason for deprescription was prolonged therapy (use for more than 12 weeks), which was observed in 59.8% of participants. The results obtained from this study highlight the necessity for a more rational use of benzodiazepines and reveal a significant percentage of nursing home residents who could benefit from the deprescription of benzodiazepines.