Inflammatory bowel disease (IBD), ulcerative colitis and Crohn's disease, are complex chronic inflammatory disorders that affect different parts of the gastrointestinal tract mucosa, with continuous changes in the state of remission and relapse. Most of the clinical symptoms are not specific to IBD and can occur in other inflammatory diseases. Pancreatic exocrine insufficiency can appear as a result of numerous disorders, and often as an extraintestinal manifestation of inflammatory bowel diseases. Fecal elastase is a pancreatic enzyme that is not degraded when passing through the intestines and correlates with the exocrine function of the pancreas. Fecal calprotectin is a protein that participates in the stimulation of the inflammatory response, and its concentrations in feces are an indirect measure of the neutrophil infiltrate in the intestinal mucosa. The aim of this work was to analyze the results of fecal elastase and fecal calprotectin in order to examine their potential in differentiating gastrointestinal disorders depending on the presence or absence of IBD. Subjects were divided into two groups (the first included patients with a diagnosis of IBD, and the second included patients with gastrointestinal diseases without IBD). From the remaining extracted stool samples of 59 subjects, the concentrations of fecal elastase and fecal calprotectin were determined by immunoturbidimetric methods on the analytical system Beckman Coulter AU 5800 (Beckman Coulter, Brea, USA). The research results showed that there were no differences in fecal elastase concentrations between patients with a diagnosis of IBD and patients with other gastrointestinal disorders. Patients with a diagnosis of IBD had high concentrations of fecal calprotectin compared to the group of patients without the mentioned disorder. Decreased fecal elastase concentrations showed a weak correlation with elevated fecal calprotectin concentrations in patients diagnosed with active IBD. Fecal calprotectin has significant diagnostic potential and can distinguish patients with IBD from patients with other gastrointestinal disorders with high sensitivity (87.88%) and specificity (88.46%). Based on the obtained results, it is concluded that fecal elastase has no additional value in the differential diagnostic approach of IBD, while fecal calprotectin has excellent diagnostic accuracy in detecting IBD.