Residential spaces affected by moisture provide ideal conditions for mold proliferation. Elevated concentrations of certain mold species can primarily cause respiratory symptoms and illnesses in individuals who spend extended periods of time in these areas. Mold isolates were recovered from indoor air sampled in moisture-affected homes and from outdoor air (control samples). Identification to the species level was conducted based on the ITS molecular marker for fungi by comparing nucleotide sequences of individual isolates with data in publicly available databases (NCBI, ISHAM). Greater biodiversity was observed among the indoor air isolates, and the following species were identified: Aspergillus aureolatus (N=1), A. jensenii (N=1), A. purpureocrustaceus (N=11), A. sulphureoviridis (N=2), A. welwitschiae/A. niger (N=1), Cladosporium cycadicola (N=2), Penicillium robsamsonii (N=5), P. robsamsonii/P.hordei (N=1), P. rubens (N=1), P. speluncae (N=4), Stachybotrys limonispora/S. chartarum (N=1), Talaromyces wortmanii (N=1), Trametes versicolor (N=1), and Verruciconidia persicana (N=1). The outdoor air isolates were identified to Beauveria pseudobassiana (N=2) and Samsoniella hepiali (N=1), while the species Ascochyta phacae (N=2), P. bialowiezense (N=5), and P. fimorum (N=10) were identified in both indoor and outdoor air samples. The results obtained indicate differences in identification depending on the NCBI or ISHAM database used and suggest updating the ISHAM database with nucleotide sequences of newly described species of the genus Penicillium and Aspergillus. The use of secondary molecular and/or chemical markers allows for precise mold identification at the species level due to overlap in nucleotide sequences in the ITS region among closely related mold species.