Von Willebrand's disease is the most common blood clotting disorder caused by a lack or pathological change of VWF. The aim of this work was to determine thrombin generation in patients with VWD using the commercially available TGA RB test on the Ceveron®s100 series analyzer (Technoclone, Vienna, Austria), that is, to determine the value of the said test in the diagnosis of VWD. Samples of 72 subjects with clinical suspicion of VWD were analyzed and divided into 5 basic groups: type 1 VWD (N=21), type 2 VWD (N=23), type 3 VWD (N=7), "low VWF" ( N=6) and patients without a proven mutation in the VWF gene (N=15). TGA RB test parameters (tLag, tPeak, Peak, VI and AUC) were compared between groups of subjects with and without VWD. The obtained results indicate the existence of a statistically significant difference in the measured parameters depending on the presence of the disease, but also on the type of disease itself. An additional analysis of the examined parameters of TGA RB was made with regard to the blood relationship of the subjects. A statistically significant difference is visible for Peak and VI between individual families. The correlation of the tested TGA RB parameters with VWF:Ag, VWF activity, APTV and FVIII activity was examined. The analysis revealed a weak or moderate to good correlation of all measured parameters with VWF:Ag, VWF activity, APTV and FVIII activity (with the exception of VWF activity and AUC). The obtained results show that the rate of thrombin generation depends on whether the person tested is suffering from VWD or is healthy, and the type of VWD affects the rate of thrombin generation. It was established that the formation of thrombin depends on the blood relationship, statistically significant differences are visible for the thrombin peak and VI between individual families. The TGA RB parameters are showing a good correlation with the parameters used in the routine diagnosis of VWD, and based on the obtained results, the TGA RB itself could be used to screen patients with VWD.