Whey proteins are among the most commonly used dietary supplements, so determining their mineral composition can be of great interest to consumers and benefit to manufacturers. The presence of heavy metals has been detected in the mineral composition of whey proteins. Considering the possible harmful effects on human health, it is useful to determine the concentration of heavy metals present. The main objective of this experimental work was to determine the elemental composition of whey proteins available on the market using the TXRF and ICP-MS techniques and to compare the results obtained with the two different techniques. The following elements were determined in all samples using both techniques: Iron, Copper, Zinc, Strontium, Rubidium. Bromine was also determined in all samples using the TXRF technique. Bromine is difficult to determine using other spectroscopic techniques, which represents a further advantage of this technique over other spectroscopic techniques for elemental analysis. The analysis of the results showed considerable differences in the element content of the different whey protein samples. Iron was the most abundant element in all samples, followed by zinc, copper and strontium. The results obtained with both techniques showed no statistically significant difference in most cases, confirming that the TXRF technique can be used as an alternative to the standardised ICP-MS technique for the determination of whey protein content Compared to the ICP-MS technique, the TXRF technique is simpler, faster, more economical and more environmentally friendly, as only a very small amount of samples and reagents are required, no cooling systems or gases are used and quantification is performed with an internal standard. The promising results obtained with this technique in the determination of heavy metal content in whey proteins show the great potential of this device for fast and easy analysis of different types of samples.