Alzheimer’s dementia (AD) is a progressive irreversible disease and the most common type of dementia today. The mechanism of the occurrence has not been confirmed, but with the collected knowledge, the most probable way is amyloid cascade hypothesis. S100B is connected with the process of neurodegeneration, in literature. It is considered as a link between amyloidosis and gliosis, two pathological processes present in AD. The main goal of this research is to determine if the value of S100B in cerebrospinal fluid differs in control group and group with AD and if those values correlate with the rest of the AD marks (t-tau, p-tau, β-amyloid).The research has been conducted on 2 groups of respondents, one controlled, while the other one consisted of respondents whose characteristics match the diagnostic criteria for AD. In both groups, the concentration of S100B has been determined in cerebrospinal fluid with electromiluminscent method by cobas 6000 cee (ECLIA, Roche Diagnostics) analyzer. The results have been processed in the statistical program MedCalc v.9.3.2.0(enabled by KZLD). Results also show that the value in cerebrospinal fluid between the two groups differ. Higher values have been found with the respondents with AD than in the controlled group, in literature, while we established lower value with the respondents with AD. The correlation made with individual marks and with their ratios is not statistically significant, however a research should be made on a larger number of samples, so that a certain confirmation can be made.