Characteristics of an ideal drug are perfectly selective biological activity, without the side effects and toxicity. For achieving that, it needs to a well define desired effect of the drug and appropriate methods to examine if it has a specific activity of this drug and how much is the efficacy and selectivity of action. Defining the desirable physical and chemical parameters in the early stages of drug research suppresses the formation of unwanted compounds in the further steps of research. Optimization of conditions is simple with the help of highthroughput technology and recently the introduction of in silico. Physico-chemical parameters of lipophilicity is the basis of observation by experts who investigate new drugs and pharmacologists, analysts, pharmaceutical technologists and other experts in the field of pharmaceutical sciences. It affects the transport of drugs through cell membranes, the interaction with the drug transport proteins, the activity and metabolism of the drug or its pharmacokinetics and pharmacodynamics. New insights into the physical and chemical parameters bioavailability febuxostat would allow a better understanding of their biological efficiency. As good choice of observations have been shown as chromatographic techniques, especially high-performance liquid chromatography with an immobilised artificial membrane which give the faithful insight into the results from the in vivo conditions. This work was carried out by HPLC with the immobilised artificial membrane for febuxostat drug that is used in the treatment of hyperuricemia. Columns, with bounded phospholipid membranes to create conditions similar to biological and predict better permeability of the drug, were used. Artificial membranes not only predict lipophilicity of drugs, but also point to the degree of interaction with phospholipids. The obtained results have shown that febuxostat is a lipophilic drug that interacts with the phospholipids or suggesting that passes through the cell membrane. Furthermore, the ADME parameters of febuxostat were investigated by using in silico approach.