The aim of this investigation was to examine daily energy intake, macronutrients consummation and dietary habits of students of the Faculty of Pharmacy and Biochemistry (University of Zagreb). Particularly, we wanted to determine the extent to which they match with dietary guidelines for proper nutrition for the mentioned population group. The study included 8 males and 21 female subjects aged 22 to 24 years. The dietetic method used in study is a four-day food diary. Filled food diaries were processed in the Excel program using the USDA charts with nutritional values for food and drink. The collected data were expressed for each gender-related subgroup. The results showed that mean daily intakes of energy and dietary fibers were lower than the recommended values. The meann dietary intakes of protein, saturated fatty acids and cholesterol were above the recommended levels. The intake of polyunsaturated fatty acids as well as the contribution of carbohydrates and fat to total caloric intake was contained within the recommendations. It has been established that there was statistically significant difference in the frequency of skipping meals between males and females. This study showed deviations from the guidelines for healthy lifestyle. It would be advisable to organize different workshops and lectures on this topic, in order to educate students about proper nutrition that complies with their physical needs.