The graduate thesis was written with the intention of investigating the understanding of the application of mathematical concepts with the students of the Physics department at the Faculty of Science at the University of Zagreb. The mathematical concepts which were inspected were integrals and gradients. The research consisted of a few steps. Firstly, through a conversation with the older students at the university, we tried to find out which mathematical concepts gave them the most trouble throughout their education at the university. Afterwards, we gave out open-type questions so we could collect as many opinions from students from different departments, and later on, offered closed-type questions, which we carried out across a larger number of respondents. The research was carried out through a conceptual exam in an online form through the platform of Microsoft Forms. When it came to integrals, we examined the conversion of problems in physics into mathematical expressions, the physical interpretation of individual mathematical symbols and expressions, and then the metric units of physical quantities. In the part of the research where we examined the understanding of the gradient, we inspected if the students knew how to numerically find the vector of the gradient at a given point. In one of the steps of the research, we inspected the proportions and the rations which remained as an introduction to further studies and research. At the end of the thesis, we suggested a way to eliminate the said difficulties.