This thesis consists of a research and methodological part. The research part describes the preparation of O-methyloxime of quinuclidin-3-one by classical synthesis and the application of mechanochemistry and microwave radiation. The compound was prepared by the reaction of hydrochloride salts of quinuclidin-3-one and methoxyamine without the addition of a base. By quaternization of the quinuclidine nitrogen atom of prepared O-methyloxime with methyl iodide, benzyl bromide and differently substituted benzyl bromides (nitro groups and halogen atoms in the meta and para positions), a series of 10 new quaternary derivatives of quinuclidine-3-one-O-methyloxime was prepared. The reactions were monitored by ATR spectroscopy. The melting points of the prepared compounds, which have not been described in the literature so far, was determined, and the structures of the obtained compounds were confirmed by mass spectrometry, infrared spectroscopy, and 1D and 2D NMR spectroscopy. The goal of the methodological part of thesis is to encourage chemistry teachers to be creative in teaching based on learning through discovery within the concept of Enzymes. The mentioned topic is an optional content in the new chemistry curriculum of the fourth grade of high school. An experiment was designed on the basis of which methodological and didactic materials were designed for one 90-minute lesson. In doing so, educational goals were set based on previous student knowledge, skills and abilities.