This research analyzed data on the functional traits of invasive plant species in Croatia and their mutual correlations, as well as the correlation of functional traits with environmental characteristics. Data were retrieved from a total of eleven databases for fifteen functional traits related to stem, leaf, flowering and seeds. An analysis of the distribution of recorded invasive species was also made with regard to the Alpine, Continental and Mediterranean biogeographical regions. Qualitative features were analyzed according to the frequency of occurrence of individual values for functional traits for Croatia, as well as for each region individually. Quantitative features were analyzed with elements of descriptive statistics, in addition to the calculated weighted mean values (corrected with regard to the frequency of findings of a particular species). Trait values among biogeographic regions were compared using ANOVA tests. The absence of established significant differences between biogeographical regions for some of the traits is partly a possible consequence of the lack of available data on traits for a larger number of species. On the other hand, the observed differences for some traits confirm the applicability of the analysis of functional traits for a better understanding of the distribution of invasive plants. By comparing the weighted mean values, the Alpine biogeographical region was shown to be different from the Continental and Mediterranean bigeographical regions.