The stone crayfish (Austropotamobius torrentium) is a native European crayfish species from family Astacidae inhabiting streams at higher altitudes with rocky bottoms and large number of shelters. The primary objective of this study was to collect and analyze data on crayfish and their habitat in order to get insight into the stone crayfish population characteristics in the natural and anthropogenicaly affected habitat, as well as to investigate wether there were differences in measured parameters (population density, morphometrical crayfish characteristics, sex ratio, body condition) between population in the natural course of the Dolje Stream and populations that are under antrophogenic influence in the downstream parts of the Dolje Stream. The crayfish were caught by hand made baited traps from June 2nd until June 9th 2015. A total of 70 crayfish were caught, of which 39 males and 31 females. For each trapped crayfish, sex, weight and seven morphometrical characteristics were recorded. Also, the body condition was determined for all individuales. Population size was estimated by Schnabel and Schumacher and Eschmeyer method. It was found that males and females differ significantly in weight, width of the first abdominal segment, size of claws and body condition. Also it was found that there were significant differences in morphometrical characteristics of stone crayfish from different localities. The biggest population size was estimated in the natural flow of the Dolje Stream where there are no anthropogenic influences, and the lowest in the canalised part of the stream, situated in the settlement. The results confirm high sensitivity of the stone crayfish to enviromental changes and indicate the great importance and necessity to preserve natural habitats of this species.