Glucosinolate that are present in the Brassicaceae family are receiving a lot of attention from researchers because of their positive properties that prevent, and cure diseases caused by today's diet and lifestyle. The aim of this work is to examine the change in the antioxidant composition and transcription factors involved in the control mechanism of lipid metabolism in mammals under the influence of commercial sinigrin. The research was conducted on 6 male and 6 female mice of the Swiss albino strain, which were given 0.3 mL or 10 mg of Sinigrin Sigma Aldrich per gram of mouse daily for a period of 28 days. The control group contained the same number of mice that received 0.3 mL of saline. One day after the last application, homogenates of liver tissue were prepared and antioxidant levels (glutathione, malondialdehyde, superoxide dismutase, catalase) and transcription factors (PPARα, PPARγ, PGC‒1α and ACOX) responsible for lipid metabolism were measured. The results show that changes related to the antioxidant response and lipid metabolism occur in the liver of male individuals with a statistically significant (ANOVA, p < 0.05) increase in malondialdehyde and catalase and a statistically significant decrease in PPARγ, PGC‒1α and ACOX1. In females, statistical significance does not occur anywhere except in the reduced levels of malondialdehyde. The exact reasons for the deviation are not known, but it is certain that glucosinolates or their products have an impact on antioxidant and metabolic processes in living beings.